LB Broth with agar (Lennox)
SIGMA/L7533 - EZMix powder microbial growth medium
Synonym: LB broth
Product Type: Chemical
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This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: L7533-1KG
| application(s) | agriculture food and beverages microbiology |
| composition | Agar, 15 g/L |
| NaCl, 5 g/L | |
| Tryptone, 10 g/L | |
| Yeast Extract, 5 g/L | |
| form | powder |
| grade | Molecular Biology |
| Quality Level | 200  |
| sterility | non-sterile |
| storage temp. | room temp |
| suitability | nonselective for Escherichia coli |
| nonselective for coliforms | |
| technique(s) | microbiological culture: suitable |
| Application: | Suitable for non-selective cultivation of E. coli strains for cloning, DNA plasmid production and production of recombinant proteins. Also suitable for selective cultivation when appropriate antibiotics are added, including those that require low-salt conditions, such as Zeocin®. |
| Features and Benefits: | Lennox EZMix powder with agar provides: • A budget-friendly alternative to pre-poured plates • Granulated, dust-free format for safer handling and faster mixing • Convenient small package to eliminate weighing • Easy scale-up using larger package sizes • Standard formulation |
| General description: | Lennox LB is a highly-referenced microbial growth medium used for the cultivation of E. coli. This nutrient-rich microbial broth contains peptides, amino acids, water-soluble vitamins, and carbohydrates in a low-salt formulation. The addition of agar provides a solid medium for microbial growth. |
| Legal Information: | Zeocin is a registered trademark of Cayla Sarl |
| Packaging: | 1 kg in poly bottle |
| Preparation Note: | 1. Suspend 35.6 g in 1 L of distilled water. 2. Heat to boiling while stirring to dissolve all ingredients completely. 3. Autoclave for 15 minutes at 121°C. To prepare Lennox L Agar: Add 1 g glucose and proceed with preparation instructions as above. To prepare the medium of Enquist and Sternberg: Aseptically add 10 ml of sterile 1 M magnesium sulfate after autoclaving. |
| Preparation Note: | Add 35.6g powder in 1L water. Heat to boiling while stirring to dissolve powder. Autoclave for 15 minutes at 121C to sterilize. Allow to cool slightly before making additions, such as antibiotics (if desired). Pour into petri dishes and allow to solidify. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 2 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | room temp |
| UNSPSC | 41106200 |