L-Lactic Dehydrogenase from porcine heart
SIGMA/L7525 - ammonium sulfate suspension, ≥200 units/mg protein
Synonym: (S)-Lactate: NAD+ oxidoreductase; L-LDH; LAD; LD; Lactate
CAS Number: 9001-60-9
EC Number: 232-617-8
MDL Number: MFCD00131466
Product Type: Chemical
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| biological source | Porcine heart |
| foreign activity | glutamic-oxalacetic transaminase ≤0.1% |
| malic dehydrogenase, glutamic-pyruvic transaminase and pyruvate kinase ≤0.06% | |
| form | ammonium sulfate suspension |
| Quality Level | 200  |
| specific activity | ≥200 units/mg protein |
| storage temp. | 2-8°C |
| UniProt accession no. | P00339  |
| Application: | L-Lactic Dehydrogenase from porcine heart has been used: • as a component of the reaction buffer to measure the GTPase activity of EngA bound to the bacterial 50S subunit to study its structure and function. • as a component of the enzyme solutions to test the sensitivity of multiphoton NAD(P)H fluorescence lifetime imaging (FLIM) in key enzymatic steps controlling the path of carbon from glucose uptake to electron transport chain (ETC) activity. |
| Biochem/physiol Actions: | Also catalyzes the oxidation of other |
| Biochem/physiol Actions: | Lactate dehydrogenase (LDH) enzyme is responsible for the conversion of pyruvate to lactate, during glycolysis under hypoxic conditions, while also converting NADH to NAD+. Moreover it acts as a crucial regulator of gluconeogenesis and DNA metabolism. Increased serum LDH levels are detected in conditions such as cancer, HIV infection, muscular dystrophy, megaloblastic anemia, extreme hypothermia, hepatitis, meningitis, hypoxia, etc. |
| General description: | Research area: Cell Signaling Lactic Dehydrogenase (LDH) is a cytoplasmic enzyme encoded by LDHA, LDHB, LDHC, and LDHD genes, and categorized as an oxidoreductase. It is widely distributed throughout the body, particularly in muscle, liver, and kidney. LDH demonstrates five isomeric forms that form tetramers with two types of subunits: muscle (M) and heart (H). |
| Other Notes: | One unit will reduce 1.0 μmole of pyruvate to |
| Physical form: | Suspension in ammonium sulfate and 0.1 M potassium phosphate, pH 7.0 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| activity | specific activity: ≥200 units/mg protein |
| Storage Temp. | 2-8°C |
| Enzyme Commission (EC) Number | 1.1.1.27 ( BRENDA | IUBMB ) |
| UNSPSC | 12352204 |