Lysozyme from chicken egg white

SIGMA/L6876 - powder or granules, ≥39,000 units/mg protein

Synonym: Mucopeptide N-acetylmuramoylhydrolase; Muramidase

CAS Number: 12650-88-3
EC Number: 235-747-3
MDL Number: MFCD00131557
Product Type: Chemical

Catalog Number	PKG Qty.	Price


45-L6876-1G	1 g	$79.30 1/EA	Add To Favorites
45-L6876-5G	5 g	$250.00 1/EA	Add To Favorites
45-L6876-10G	10 g	$331.00 1/EA	Add To Favorites
45-L6876-25G	25 g	$746.00 1/EA	Add To Favorites
45-L6876-100G	100 g	$2660.00 1/EA	Add To Favorites

Lysozyme specificity: Peptidoglycans are polymers of β-(1-4)-N-Acetyl-D-glucosamine units. Alternating residues are modified to form N-acetylmuramic acid with the addition of lactate to form branching links to the tetrapeptide.

This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: L6876-10G

Properties
Descriptions
Safety Info.
Basic Data

application(s)	cell analysis
biological source	chicken egg white
form	powder or granules
mol wt	single-chain 14.3 kDa
Quality Level	200
specific activity	≥39,000 units/mg protein
storage temp.	room temp
suitability	suitable for cell lysis
technique(s)	cell based assay: suitable
UniProt accession no.	P00698

Application:	Enzyme breaks down the cell walls of bacteria; used to prepare spheroplasts.
Application:	Lysozyme from chicken egg white has been used for the extraction of genomic DNA from bacterial cells. It has been used as an external standard for MALDI-TOF (matrix assisted laser desorption ionization-time of flight) mass analysis.
Biochem/physiol Actions:	Lysozyme hydrolyzes β(1→4) linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues in peptidoglycan and between N-acetyl-D-glucosamine residues in chitodextrin. Gram-positive cells are quite susceptible to this hydrolysis as their cell walls have a high proportion of peptidoglycan.
Biochem/physiol Actions:	Lysozyme hydrolyzes β(1→4) linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues in peptidoglycan and between N-acetyl-D-glucosamine residues in chitodextrin. Gram-positive cells are quite susceptible to this hydrolysis as their cell walls have a high proportion of peptidoglycan. Gram-negative bacteria are less susceptible due to the presence of an outer membrane and a lower proportion of peptidoglycan. However, these cells may be hydrolyzed in the presence of EDTA that chelates metal ions in the outer bacterial membrane. The enzyme is active over a broad pH range (6.0 to 9.0). At pH 6.2, maximal activity is observed over a wider range of ionic strengths (0.02 to 0.100 M) than at pH 9.2 (0.01 to 0.06 M).
Features and Benefits:	Lysozyme from chicken egg white is a versatile enzyme with unique properties that make it effective for various applications, including bacterial cell wall breakdown, genomic DNA extraction, and external standard for mass analysis. It is reliable, active over a broad pH range, and available in various packaging sizes.
Other Notes:	One unit will lyse 0.6 μg of Micrococcus lysodeikticus per minute by turbidimetric detection at 600 nm when suspended in buffer at pH 6.2 at 25 °C.
Other Notes:	View more information on enzymes for complex carbohydrate analysis at www.sigma-aldrich.com/enzymeexplorer
Packaging:	1, 5, 10, 25, 100 g in poly bottle

Symbol	GHS08
Signal word	Danger
Hazard statements	H334
Precautionary statements	P261 - P284 - P501
RIDADR	NONH for all modes of transport
WGK Germany	WGK 3
Flash Point(F)	Not applicable
Flash Point(C)	Not applicable

activity	specific activity: ≥39,000 units/mg protein
Storage Temp.	room temp
Enzyme Commission (EC) Number	3.2.1.17 ( BRENDA \| IUBMB )
UNSPSC	12352204

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