D-Luciferin
SIGMA/L6152 - synthetic, BioXtra, ≥99% (HPLC)
Synonym: (S)
CAS Number: 2591-17-5
Empirical Formula (Hill Notation): C11H8N2O3S2
Molecular Weight: 280.32
EC Number: 219-981-3
MDL Number: MFCD00042929
Linear Formula: C11H8N2O3S2
Product Type: Chemical
ATP is hydrolyzed and light is emitted when firefly luciferase catalyzes the oxidation of D-luciferin. Reaction (1) is reversible and the equilibrium lies far to the right. Reaction (2) is essentially irreversible. When ATP is the limiting reagent, the light emitted is proportional to the ATP present.
| assay | ≥99% (HPLC) |
| biological source | synthetic |
| form | powder |
| InChI | 1S/C11H8N2O3S2/c14-5-1-2- |
| InChI key | BJGNCJDXODQBOB-SSDOTTSWSA |
| product line | BioXtra |
| Quality Level | 200  |
| SMILES string | OC(=O)[C@H]1CSC(=N1)c2nc3 |
| solubility | DMSO: 10 mg/mL, clear, colorless to faintly yellow |
| storage temp. | −20°C |
| Application: | D-Luciferin has been used in the preparation of the reaction mixture for nitric oxide determination by minescence measurement. It has also been used in the preparation of D-luciferin stock solution for luciferin assays. |
| Application: | Substrate for firefly luciferase with a Km of approx 2 μM. |
| General description: | D-Luciferin is mainly produced in bioluminescent organisms. It has the ability to simply circulate across the blood testis barrier, blood brain barrier and the blood placenta barrier. This small molecule is sensitive to light and oxygen. It is also sensitive to moisture in its powder form. |
| Other Notes: | Highly purified grade with reduced levels of inhibitor relative to L 9504 |
| Packaging: | 1, 10 mg in glass bottle |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | ≥99% (HPLC) |
| Storage Temp. | −20°C |
| UNSPSC | 12352204 |