Lipopolysaccharides from Salmonella enterica serotype typhimurium
SIGMA/L6143 - BioXtra, suitable for cell culture, γ-irradiated
Synonym: LPS
MDL Number: MFCD00164404
Product Type: Chemical
Lipopolysaccharides (LPS) are localized in the outer layer of the membrane and are, in noncapsulated strains, exposed on the cell surface. They contribute to the integrity of the outer membrane, and protect the cell against the action of bile salts and lipophilic antibiotics.
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: L6143-1MG
| biological source | Salmonella enterica (serotype typhimurium) |
| form | lyophilized powder |
| impurities | <1% Protein (Lowry) |
| product line | BioXtra |
| purified by | gel-filtration chromatography |
| Quality Level | 200  |
| sterility | γ-irradiated |
| storage temp. | 2-8°C |
| technique(s) | cell culture | mammalian: suitable |
| Application: | Lipopolysaccharides (LPSs) are characteristic components of the cell wall of Gram-negative bacteria. LPS and its lipid A moiety stimulate cells of the innate immune system by the Toll-like receptor 4 (TLR4), a member of the Toll-like receptor protein family, which recognizes common pathogen-associated molecular-patterns (PAMPs). |
| Application: | Lipopolysaccharides from Salmonella enterica serotype typhimurium has been used: • in mice to elicit the secretion of pro-inflammatory cytokines • to induce cytokine production in monocytes • to elicit the activation of NF-κB pathway in mouse dendritic cells. • to induce inflammatory response in human colon adenocarcinoma Caco-2 cells • for stimulation of the catfish peripheral blood leukocytes (PBLs) to study estrogen receptor mRNA expression |
| Biochem/physiol Actions: | LPS is a major constituent of the cell wall of most gram negative bacteria. It is a highly immunogenic antigen with the ability to enhance immune responses to soluble antigens. LPS also acts as a specific mitogen for bone marrow derived B lymphocytes from mice, rabbits, chickens, cows, hamsters, and humans. |
| Other Notes: | To gain a comprehensive understanding of our extensive range of Lipopolysaccharides for your research, we encourage you to visit our Carbohydrates Category page. |
| Packaging: | 1 mg in glass bottle |
| Preparation Note: | Lipopolysaccharides are supplied as lyophilized, γ-irradiated powders. To reconstitute, add 1 ml sterile balanced salt solution or tissue culture medium to the vial (1 mg) and gently swirl until the powder dissolves. Reconstituted product may be further diluted to desired working concentrations using sterile balanced salt solution or tissue culture medium. |
| Symbol |  GHS07 |
| Signal word | Warning |
| Hazard statements | H302 + H312 + H332 |
| Precautionary statements | P261 - P264 - P280 - P301 + P312 - P302 + P352 + P312 - P304 + P340 + P312 |
| Hazard Codes | Xn |
| Risk Statements | 20-21-22 |
| Safety Statements | 36 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | 2-8°C |
| UNSPSC | 12352201 |