Lectin from Arachis hypogaea (peanut)
SIGMA/L6135 - biotin conjugate, lyophilized powder
Synonym: PNA; Peanut agglutinin
Product Type: Chemical
Agglutination assay: Lectin from Arachis hypogaea (peanut) (Cat. No. L6135) at a concentration of ~1mg solid/mL was serially diluted (2-fold, top to bottom of plate) and incubated with human erythrocytes type: O (4% v/v RBC) to calculate Minimum agglutination titer (MAT). Agglutination is defined as the inability of cells to form a tight button. PBS was used as blank.
| composition | Protein, ≥80% Lowry |
| conjugate | biotin conjugate |
| extent of labeling | ~3 mol biotin per mol protein |
| form | lyophilized powder |
| mol wt | 120 kDa |
| Quality Level | 200  |
| solubility | water: 1.00-1.10 mg/mL, clear to slightly hazy, colorless |
| storage temp. | 2-8°C |
| technique(s) | blood typing: suitable |
| fractionation: suitable |
| Application: | Lectin from Arachis hypogaea (peanut) has been used: • in enzyme-linked micro plate assay to detect β- • in Immunohistochemical double staining • Immunohistochemical double staining for the detection of germinal centers • in enzyme-linked microplate assayto detect β- |
| Biochem/physiol Actions: | Lectin from Arachis hypogaea (peanut) functions as an antigenic determinant for Thomsen-Friedenreich antigen (TF) and as mitogen for colorectal cancer stimulation cell division in normal colonic epithelium. It is therefore used as tumor marker. |
| Biochem/physiol Actions: | PNA does not agglutinate normal human erythrocytes, but strongly agglutinates neuraminidase treated erythrocytes. PNA has potent anti-T activity similar to the anti-T antibody in human sera. The lectin can be used to distinguish between human lymphocyte subsets. |
| General description: | Peanut lectin (PNA) is a lectin or an agglutinin from the legume, Arachis hypogaea, found in peripheral venous blood in humans after ingestion of peanuts. PNA lectin is specific to the galactosyl (β-1,3) N-acetylgalactosamine. |
| Packaging: | 1, 5 mg in serum bottle |
| Packaging: | Package size based on protein content. |
| Physical form: | Contains sodium citrate |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | 2-8°C |
| UNSPSC | 12352202 |