α-Lactalbumin from bovine milk
SIGMA/L6010 - Type III, calcium depleted, ≥85% (PAGE), lyophilized powder
Synonym: Bos d 4; Lactose synthase B protein; alpha-lactalbumin
CAS Number: 9051-29-0
MDL Number: MFCD00162025
Product Type: Chemical
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: L6010-100MG
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: L6010-1G
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: L6010-500MG
| assay | ≥85% (PAGE) |
| biological source | bovine milk |
| cation traces | Ca: ≤0.3 mol/mol |
| concentration | ≥85 % protein |
| form | lyophilized powder |
| impurities | cation traces, tested |
| mol wt | 14,178 Da by calculation |
| quality | calcium depleted |
| Quality Level | 300  |
| solubility | H2O: soluble 10 mg/mL, clear to slightly hazy, colorless to faintly yellow |
| storage temp. | −20°C |
| technique(s) | indirect ELISA: suitable |
| type | Type III |
| UniProt accession no. | P00711  |
| Analysis Note: | May contain traces of (NH4)2SO4 and sodium phosphate. |
| Application: | α-Lactalbumin from bovine milk has been used: • in indirect enzyme-linked immunosorbent assay (ELISA) and competitive ELISA methods • in binding of Hsp90 and HSJ1b analysis • to inhibit the lysozyme CAP-RAST assay |
| Biochem/physiol Actions: | Alters the substrate specificity of galactosyltransferase to increase the rate of lactose formation; the complex of galactosyltransferase and α-lactalbumin is called lactose synthase. |
| Biochem/physiol Actions: | Alters the substrate specificity of galactosyltransferase to increase the rate of lactose formation; the complex of galactosyltransferase and α-lactalbumin is called lactose synthase. Site-directed mutagenesis of Asp87 or Asp88 to Ala completely abolishes the strong calcium binding affinity and reduces the stimulation of lactose synthase to <3.5% of the maximal rate. |
| Biochem/physiol Actions: | α-Lactalbumin is the cheif protein in human milk. It consists of a single polypeptide chain with 8 cysteines which form disulfide bridges. α-Lactalbumin binds several metal ions, including calcium, which is thought to play a role in the regeneration of native α-lactalbumin from the reduced, denatured form. α-Lactalbumin also has a distinct zinc binding site that is thought to play a role in the binding of the lactose synthase complex.The mature protein consists of 123 amino acid residues (14 kD), and it has a three-dimensional structure with 1.7 Angstrom resolution, demonstrating four α-helices and a triple stranded antiparallel β-sheet. |
| General description: | may contain traces of (NH4)2SO4 and sodium phosphateL6010 |
| Packaging: | 1 g in poly bottle |
| Packaging: | 100, 500 mg in poly bottle |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | ≥85% (PAGE) |
| Storage Temp. | −20°C |
| UNSPSC | 12352202 |