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Leucine Aminopeptidase, microsomal from porcine kidney

SIGMA/L6007 - Type VI-S, lyophilized powder, ≥12 units/mg protein (biuret)

Synonym: Aminopeptidase M; Aminopeptidase N; Cathepsin III; LAP; Leucinamide aminopeptidase; Leucinaminopeptidase

CAS Number: 9054-63-1
EC Number: 232-942-5
MDL Number: MFCD00131501
Product Type: Chemical
Catalog Number PKG Qty. Price Quantity
45-L6007-25UN 25 units
 $567.00
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45-L6007-50UN 50 units
 $887.00
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45-L6007-100UN 100 units
 $1620.00
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This image is provided for informative purposes only and does not represent an actual product label. It should not be used as a substitute for official product labeling.

 

biological source Porcine kidney
composition Protein, 30-70%
form lyophilized powder
mol wt 320 kDa
Quality Level 200 
specific activity ≥12 units/mg protein (biuret)
storage temp. −20°C
type Type VI-S
Application: Leucine Aminopeptidase, microsomal from porcine kidney has been used in aminopeptidase N (APN) inhibitor screening assay in adenocarcinoma cell line ES-2 and in the inhibition kinetic studies with APN inhibitor.
Biochem/physiol Actions: Leucine Aminopeptidase (LAP) catalyzes protein hydrolysis at the leucine residue at N-terminal. An altered level of LAP is implicated in tumor proliferation and progression. LAP is a potential biomarker for ovarian epithelial cancer therapy. Porcine LAP hydrolyzes glycine and arginine substrates.
General description: Leucine Aminopeptidase (LAP) from porcine is a 320-kDa exopeptidase present in cytosol. It contains zinc in its structure and belongs to the M1 and M17 peptidase families. LAP display bi-lobed structure and has the catalytic domain at the C-terminus and a variable N-terminal domain.
Other Notes: One unit will hydrolyze 1.0 μmole of L-leucine-p-nitroanilide to L-leucine and p-nitroaniline per min at pH 7.2 at 37 °C. (At 25 °C, approx. 40% of the activity at 37 °C is obtained.) The activity obtained using L-leucine p-nitroanilide as substrate is 2-5 times that obtained with L-leucinamide as substrate.
Packaging: 25, 50, 100 units in glass bottle
Physical form: Contains primarily phosphate buffer salts, pH 7.0
activity specific activity: ≥12 units/mg protein (biuret)
Storage Temp. −20°C
Enzyme Commission (EC) Number 3.4.11.2   ( BRENDA  | IUBMB  )
UNSPSC 12352204

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