Lectin from Ulex europaeus (gorse, furze)
SIGMA/L5505 - lyophilized powder
Synonym: Ulex europaeus agglutinin; UEA-I
Product Type: Chemical
Agglutination
assay: Lectin from Ulex europaeus (gorse, furze) (Cat.
No. L5505) at a concentration of ~1mg solid/mL was serially diluted (2-fold,
top to bottom of plate) and incubated with human erythrocytes type: O (4% v/v
RBC) to calculate Minimum agglutination titer (MAT). Agglutination is defined
as the inability of cells to form a tight button. PBS was used as blank.
assay: Lectin from Ulex europaeus (gorse, furze) (Cat.
No. L5505) at a concentration of ~1mg solid/mL was serially diluted (2-fold,
top to bottom of plate) and incubated with human erythrocytes type: O (4% v/v
RBC) to calculate Minimum agglutination titer (MAT). Agglutination is defined
as the inability of cells to form a tight button. PBS was used as blank.
SDS-PAGE Lectin from Ulex europaeus (gorse, furze) (Cat. No. L5505) was assessed on SDS-PAGE.
| composition | Protein, ≥80% biuret |
| form | lyophilized powder |
| impurities | salt, essentially free |
| potency | <8 μg/mL agglutination activity |
| Quality Level | 200  |
| storage temp. | 2-8°C |
| Analysis Note: | Agglutination activity is expressed in μg/ml and is determined from serial dilutions in phosphate buffered saline, pH 6.8, of a 1 mg/ml solution. This activity is the lowest concentration to agglutinate a 2% suspension of human blood group O erythrocytes after 1 hour incubation at 25 °C. |
| Biochem/physiol Actions: | Ulex europaeus agglutinin has anti-H blood group specificity. There are two types of lectin: UEA I has an affinity for |
| Other Notes: | Conjugates are prepared from affinity purified lectin. |
| Packaging: | 2, 5 mg in glass bottle |
| Preparation Note: | Purified by affinity chromatography using the method of Pereira. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | 2-8°C |
| UNSPSC | 12352202 |