Lectin from Bandeiraea simplicifolia (Griffonia simplicifolia)
SIGMA/L5391 - Isolectin B4 (BSI-B4), peroxidase conjugate, lyophilized powder
Synonym: Bandeirea simplicifolia agglutinin; BS-I
Product Type: Chemical
Agglutination
assay: Lectin from Bandeiraea simplicifolia (Griffonia
simplicifolia) (Cat. No. L5391) at a concentration of ~1mg solid/mL was
serially diluted (2-fold, top to bottom of plate) and incubated with human
erythrocytes type: B (4% v/v RBC) to calculate Minimum agglutination titer
(MAT). Agglutination is defined as the inability of cells to form a tight
button. PBS was used as blank.
assay: Lectin from Bandeiraea simplicifolia (Griffonia
simplicifolia) (Cat. No. L5391) at a concentration of ~1mg solid/mL was
serially diluted (2-fold, top to bottom of plate) and incubated with human
erythrocytes type: B (4% v/v RBC) to calculate Minimum agglutination titer
(MAT). Agglutination is defined as the inability of cells to form a tight
button. PBS was used as blank.
Lanes 1. Urokinase from human urine (Sigma U0633) 2. Factor IX Human (Sigma F0806) 3. Complement component C1q from human serum (Sigma C1740) (galNAc, Gal) 4. a1-Antitrypsin from human plasma (Sigma A3106) 5. Fetuin (Sigma F2379) (glcNAc) 6. Invertase glycoprotein Standard (Sigma I0408) 7. IgG1 Monoclonal Anti-Flag M2 Ab produced in mouse (Sigma F3165) (Gal, Man, glcNac, Fuc) 8. IgG2a Monoclonal Anti-AMY1A Ab produced in mouse (Sigma WH0000276M4) (Gal, Man, glcNac, Fuc) 9. Recombinant Human t-Plasminogen Activator/tPA (R&D Systems 7449-SE-010) (glcNAc, Fuc) 10. Epidermal growth factor receptor human (Sigma E3641) 11. Apolipoprotein C-III from human plasma (Sigma A3106) 12. Thrombospondin from human platelets (Calbiochem 605225)
| assay | ≥90% protein basis (Warburg-Christian) |
| biological source | Bandieraea simplicifolia |
| composition | Protein, ~95% modified Warburg-Christian |
| conjugate | peroxidase conjugate |
| form | lyophilized powder |
| peroxidase activity | 40-160 units/mg protein |
| potency | <200 μg per mL agglutination activity (using human blood group B erythrocytes) |
| Quality Level | 200  |
| storage temp. | −20°C |
| technique(s) | blood typing: suitable |
| fractionation: suitable |
| Analysis Note: | Agglutination activity is expressed in μg/mL and is determined from serial dilutions of a 1 mg/mL solution using phosphate buffered saline, pH 6.8, containing, for each lectin, calcium, magnesium, and manganese at different concentrations. This activity is the lowest concentration to agglutinate a 2% suspension of appropriate erythrocytes after 1 hr incubation at 25 °C. |
| Application: | General Western Blot Protocol: • Glycoprotein sample size: 500ng • Lectin Concentration: 0.1ug/ml
|
| Biochem/physiol Actions: | BS-I has a major affinity for terminal α- |
| Other Notes: | BS-I is a tetrameric lectin consisting of two types of subunits designated A and B. There are five BS-I isolectins with different subunit composition: BSI-B4, BSI-AB3, BSI-A2B2, BSI-A3B and BSI-A4. BSI-B4 is blood group B specific and has an exclusive affinity for terminal α- |
| Other Notes: | One unit will form 1 mg purpurogallin in 20 sec from pyrogallol at pH 6.0 at 20 °C. |
| Physical form: | Contains sodium citrate buffer salts and calcium chloride |
| Preparation Note: | Prepared from peroxidase type VI using a modification of the method of O′Sullivan, et al. Repurified by affinity chromatography after conjugation. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | ≥90% protein basis (Warburg-Christian) |
| activity | peroxidase activity: 40-160 units/mg protein |
| Storage Temp. | −20°C |
| UNSPSC | 12352202 |