α-Lactalbumin from bovine milk
SIGMA/L5385 - Type I, ≥85% (PAGE), lyophilized powder
Synonym: Bos d 4; Lactose synthase B protein; alpha-lactalbumin
CAS Number: 9051-29-0
MDL Number: MFCD00162025
Product Type: Chemical
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: L5385-100MG
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: L5385-25MG
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: L5385-1G
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: L5385-500MG
| assay | ≥85% (PAGE) |
| biological source | bovine milk |
| concentration | ≥85 % protein |
| form | lyophilized powder |
| impurities | calcium, tested |
| Quality Level | 300  |
| solubility | H2O: soluble 10 mg/mL, clear to slightly hazy, colorless to faintly yellow |
| storage temp. | −20°C |
| technique(s) | cell culture | mammalian: suitable |
| electrophoresis: suitable | |
| type | Type I |
| UniProt accession no. | P00711  |
| Analysis Note: | Calcium saturated. May have traces of ammonium sulfate and sodium phosphate |
| Application: | α-Lactalbumin from bovine milk has been used as a supplement of basal medium for various cell cultures. It has also been used as a marker for sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). |
| Biochem/physiol Actions: | Alters the substrate specificity of galactosyltransferase to increase the rate of lactose formation; the complex of galactosyltransferase and α-lactalbumin is called lactose synthase. |
| Biochem/physiol Actions: | Alters the substrate specificity of galactosyltransferase to increase the rate of lactose formation; the complex of galactosyltransferase and α-lactalbumin is called lactose synthase. Site-directed mutagenesis of Asp87 or Asp88 to Ala completely abolishes the strong calcium binding affinity and reduces the stimulation of lactose synthase to <3.5% of the maximal rate. |
| Biochem/physiol Actions: | α-Lactalbumin is the cheif protein in human milk. It consists of a single polypeptide chain with 8 cysteines which form disulfide bridges. α-Lactalbumin binds several metal ions, including calcium, which is thought to play a role in the regeneration of native α-lactalbumin from the reduced, denatured form. α-Lactalbumin also has a distinct zinc binding site that is thought to play a role in the binding of the lactose synthase complex. The mature protein consists of 123 amino acid residues (14 kD), and it has a three-dimensional structure with 1.7 Α° resolution, demonstrating four α-helices and a triple stranded antiparallel β-sheet. |
| General description: | α-Lactalbumin is a small, globular, whey protein that has been found in all milk studied to date. It is a metalloprotein of approximately 14 kDa produced in the mammary glands. |
| Packaging: | 1 g in poly bottle |
| Packaging: | 25, 100, 500 mg in poly bottle |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | ≥85% (PAGE) |
| Storage Temp. | −20°C |
| UNSPSC | 12352202 |