Lipopolysaccharides from Escherichia coli O111:B4
SIGMA/L5293 - Ready Made solution, 1 mg/mL
Synonym: LPS
CAS Number: 93572-42-0
MDL Number: MFCD00164401
Product Type: Chemical
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| biological source | Escherichia coli (O111:B4) |
| color | colorless to faint yellow |
| concentration | 1 mg/mL |
| form | aqueous solution |
| Quality Level | 200  |
| shipped in | wet ice |
| solubility | water: soluble |
| storage temp. | 2-8°C |
| Application: | Lipopolysaccharides (LPSs) are characteristic components of the cell wall of Gram-negative bacteria. LPS and its lipid A moiety stimulate cells of the innate immune system by the Toll-like receptor 4 (TLR4), a member of the Toll-like receptor protein family, which recognizes common pathogen-associated molecular-patterns (PAMPs). |
| Application: | Lipopolysaccharides from Escherichia coli O111:B4 has been used: • to stimulate an inflammatory response in porcine liver tissues in-vivo and piglet ileum tissues ex vivo • to stimulate Ex vivo natural killer(NK) cells and hepatic B cells • to induce arthritis in a standard rabbit model |
| Biochem/physiol Actions: | Lipopolysaccharides (LPS) are distributed on the outer layer of the membrane and in non-capsulated strains, present on the cell surface. LPS maintains the outer membrane integrity. In addition, it protects the cell from bile salts stress and lipophilic antibiotics. |
| Other Notes: | To gain a comprehensive understanding of our extensive range of Lipopolysaccharides for your research, we encourage you to visit our Carbohydrates Category page. |
| Preparation Note: | The product is soluble in water (5 mg/ml) or cell culture medium (1 mg/ml) yielding a hazy, faint yellow solution. A more concentrated, though still hazy, solution (20 mg/ml) has been achieved in aqueous saline after vortexing and warming to 70-80 oC. Lipopolysaccharides are molecules that form micelles in every solvent. Hazy solutions are observed in water and phosphate buffered saline. Organic solvents do not give clearer solutions. Methanol yields a turbid suspension with floaters, while water yields a homogeneously hazy solution. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | 2-8°C |
| UNSPSC | 12352201 |