Leukemia Inhibitory Factor from mouse
SIGMA/L5158 - LIF, recombinant, expressed in E. coli, 10 μg/ml, buffered aqueous solution, suitable for cell culture
Synonym: Mouse LIF; Mouse Leukemia Inhibitory Factor; Murine LIF; mLIF; LIF
MDL Number: MFCD00283681
Product Type: Chemical
SDS-PAGE Leukemia Inhibitory Factor from mouse (Cat. No. L5158) was assessed on SDS-PAGE.
Bioassay EC50 is defined as the effective concentration of LIF that elicits a 50% attachment factor in cell based bioassay.
| assay | ≥95% (SDS-PAGE) |
| form | buffered aqueous solution |
| quality | endotoxin tested |
| Quality Level | 200  |
| recombinant | expressed in E. coli |
| storage temp. | 2-8°C |
| technique(s) | cell culture | mammalian: suitable |
| Analysis Note: | The proliferative activity of mouse LIF is measured in culture using the M1 murine cell line. |
| Application: | Leukemia Inhibitory Factor from mouse has been used: • as a component of enriched co-culture media • as a supplement in Dulbecco′s modified eagle′s medium (DMEM)/F12 medium for culturing heart cells • as a component in maintenance medium for maintaining embryonic stem cells • to treat ovaries in ovarian culture |
| Biochem/physiol Actions: | Leukemia Inhibitory Factor (LIF) is a pleiotropic glycoprotein originally described to inhibit the proliferation of the murine myeloid leukemic cell line M1, while inducing differentiation into macrophages. Other activities were later identified to LIF, which is known by a variety of synonyms, including DIF, D-factor, DIA, DRF, CNDF, HILDA, HSF-III, and MLPLI. Human LIF exerts its actions through a receptor comprising a 190 kDa LIF-binding α-chain (130 kDa, mouse) and a 130 kDa signal-transducing β-chain (gp130), which is shared with CNTF, OSM, L-6 and IL-11. As such, it is a member of the gp130 family of the cytokine receptor superfamily. LIF receptors have been identified on several cells, including monocytes, liver, placenta and embryonic stem cells. Natural LIF is heavily glycosylated, showing an apparent molecular weight of 32 kDa to 62 kDa, depending on the source, but absence of glycosylation appears not to affect its biological activity. A single gene encodes LIF, which is secreted as a single chain glycoprotein containing 180 amino acids for human or mouse with a conserved disulfide bond. Human and mouse LIF share 78% sequence homology. Human LIF can activate mouse cells, but mouse LIF cannot activate human cells. |
| Biochem/physiol Actions: | Leukemia Inhibitory Factor (LIF) is a pleiotropic glycoprotein that inhibits the proliferation of the murine myeloid leukemic cell line M1, while inducing differentiation into macrophages. Human and mouse LIF share 78% sequence homology. Human LIF can activate mouse cells, but mouse LIF cannot activate human cells. |
| Legal Information: | TWEEN is a registered trademark of Croda International PLC |
| Physical form: | Solution in phosphate buffered saline containing 0.02% TWEEN® 20 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | ≥95% (SDS-PAGE) |
| Storage Temp. | 2-8°C |
| UNSPSC | 12352202 |