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Leucine Aminopeptidase, microsomal from porcine kidney

SIGMA/L5006 - Type IV-S, ammonium sulfate suspension, 10-40 units/mg protein (Bradford)

Synonym: Aminopeptidase M; Aminopeptidase N; Cathepsin III; LAP; Leucinamide aminopeptidase; Leucinaminopeptidase

CAS Number: 9054-63-1
EC Number: 232-942-5
MDL Number: MFCD00131501
Product Type: Chemical
Catalog Number PKG Qty. Price Quantity
45-L5006-25UN 25 units
 $172.00
1/EA		 Add To Favorites
45-L5006-50UN 50 units
 $301.00
1/EA		 Add To Favorites
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form ammonium sulfate suspension
Quality Level 200 
specific activity 10-40 units/mg protein (Bradford)
storage temp. 2-8°C
type Type IV-S
Application: Leucine Aminopeptidase, microsomal from porcine kidney has been used in the isolation of neprilysin (NEP) from rodent brain-C5 and for peptide digestion.
Biochem/physiol Actions: Leucine Aminopeptidase possesses broad substrate-specificity. It hydrolyzes peptides up to the proline residue and does not continue to degrade beyond proline.
General description: Leucine Aminopeptidase belongs to the family of metalloprotease.
Other Notes: One unit will hydrolyze 1.0 μmole of L-leucine-p-nitroanilide to L-leucine and p-nitroaniline per min at pH 7.2 at 37 °C. (At 25 °C, approx. 40% of the activity at 37 °C is obtained.) The activity obtained using L-leucine p-nitroanilide as substrate is 2-5 times that obtained with L-leucinamide as substrate.
Physical form: Suspension in 3.5 M (NH4)2SO4 solution, pH 7.7, containing 10 mM MgCl2
activity specific activity: 10-40 units/mg protein (Bradford)
Storage Temp. 2-8°C
Enzyme Commission (EC) Number 3.4.11.2   ( BRENDA  | IUBMB  )
UNSPSC 12352204

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