Lysozyme from chicken egg white
SIGMA/L4919 - BioUltra, lyophilized powder, ≥98% (SDS-PAGE), ≥40,000 units/mg protein
Synonym: Mucopeptide N-
CAS Number: 12650-88-3
EC Number: 235-747-3
MDL Number: MFCD00131557
Product Type: Chemical
Lysozyme specificity: Peptidoglycans are polymers of β-(1-4)-N-Acetyl-D-glucosamine units. Alternating residues are modified to form N-acetylmuramic acid with the addition of lactate to form branching links to the tetrapeptide.
SDS-PAGE (4-20%) of lysozyme (Cat. No. L4919) under reducing conditions and stained with Coomassie Blue.
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: L4919-1G
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: L4919-500MG
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: L4919-5G
| application(s) | cell analysis |
| assay | ≥98% (SDS-PAGE) |
| biological source | chicken egg white |
| composition | Protein, ≥90% |
| form | lyophilized powder |
| grade | Molecular Biology |
| mol wt | single-chain 14.3 kDa |
| product line | BioUltra |
| Quality Level | 200  |
| specific activity | ≥40,000 units/mg protein |
| storage temp. | −20°C |
| suitability | suitable for cell lysis |
| technique(s) | cell based assay: suitable |
| Application: | Enzyme breaks down the cell walls of bacteria; used to prepare spheroplasts. |
| Application: | Lysozyme from chicken egg white has been used: • in dielectric spectroscopy studies of dynamics of protein • as a control to measure the human lysozyme activity • as a supplement in soaking solution to treat lenses |
| Biochem/physiol Actions: | Lysozyme hydrolyzes β(1→4) linkages between N-acetylmuramic acid and N-acetyl- |
| Biochem/physiol Actions: | Lysozyme hydrolyzes β(1→4) linkages between N-acetylmuramic acid and N-acetyl- The enzyme is active over a broad pH range (6.0 to 9.0). At pH 6.2, maximal activity is observed over a wider range of ionic strengths (0.02 to 0.100 M) than at pH 9.2 (0.01 to 0.06 M). |
| Biochem/physiol Actions: | Lysozymes participate in the defense mechanism. It has the ability to stimulate catalysis by bringing steric stress in the substrates. |
| Features and Benefits: | • Highly purified by repeated crystallization and dialysis • Each lot is use-tested for isolation of plasmid DNA from E. coli |
| General description: | Lysozyme is abundantly found in animal and plant kingdoms. It is a natural food preservative and also found to be present in specific bacterial cell walls. Lysozyme is usually present in tears, milk, urine and saliva. |
| Other Notes: | One unit will lyse 0.6 μg of Micrococcus lysodeikticus per minute by turbidimetric detection at 600 nm when suspended in buffer at pH 6.2 at 25 °C. |
| Packaging: | 1, 5 g in poly bottle |
| Packaging: | 500 mg in poly bottle |
| Preparation Note: | 3× crystallized |
| Symbol |  GHS08 |
| Signal word | Danger |
| Hazard statements | H334 |
| Precautionary statements | P261 - P284 - P501 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | ≥98% (SDS-PAGE) |
| activity | specific activity: ≥40,000 units/mg protein |
| Storage Temp. | −20°C |
| Enzyme Commission (EC) Number | 3.2.1.17 ( BRENDA  | IUBMB ) |
| UNSPSC | 12352204 |