Lipopolysaccharides from Escherichia coli O111:B4
SIGMA/L4391 - γ-irradiated, BioXtra, suitable for cell culture
Synonym: LPS
CAS Number: 93572-42-0
EC Number: 297-473-0
MDL Number: MFCD00164401
Product Type: Chemical
Lipopolysaccharides (LPS) are localized in the outer layer of the membrane and are, in noncapsulated strains, exposed on the cell surface. They contribute to the integrity of the outer membrane, and protect the cell against the action of bile salts and lipophilic antibiotics.
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: L4391-1MG
| application(s) | clinical research life science and biopharma |
| biological source | Escherichia coli (O111:B4) |
| form | lyophilized powder |
| impurities | <1% Protein (Lowry) |
| product line | BioXtra |
| purified by | gel-filtration chromatography |
| Quality Level | 300  |
| solubility | H2O: 5 mg/mL, slightly hazy |
| sterility | γ-irradiated |
| storage condition | desiccated |
| storage temp. | 2-8°C |
| technique(s) | cell based assay: suitable |
| cell culture | mammalian: suitable |
| Application: | Lipopolysaccharides (LPSs) are characteristic components of the cell wall of Gram-negative bacteria. LPS and its lipid A moiety stimulate cells of the innate immune system by the Toll-like receptor 4 (TLR4), a member of the Toll-like receptor protein family, which recognizes common pathogen-associated molecular-patterns (PAMPs). |
| Application: | This LPS serotype has been used to stimulate B-cells and induce NOS in human hepatocytes. |
| Biochem/physiol Actions: | LPS is a major constituent of the cell wall of most gram negative bacteria. It is a highly immunogenic antigen with the ability to enhance immune responses to soluble antigens. LPS also acts as a specific mitogen for bone marrow derived B lymphocytes from mice, rabbits, chickens, cows, hamsters, and humans. |
| Features and Benefits: | • High-purity compound suitable for a wide variety of research applications |
| General description: | Lipopolysaccharides (LPS) are intricate molecules consisting of lipids and polysaccharides, predominantly present in the outer membrane of Gram-negative bacteria. Comprising lipid A, the core oligosaccharide (core), and the O-specific polysaccharide or O antigen (OAg), LPS is anchored to the outer bacterial membrane, with lipid A primarily responsible for its associated toxicity. Functioning as a potent activator of the proinflammatory response, LPS engages various mammalian cells, including macrophages, monocytes, and endothelial cells. While serving a protective role for Gram-negative bacteria against environmental stress, LPS also triggers recognition by the innate immune system during infections. |
| Other Notes: | For additional information on our range of Biochemicals , please complete this form . |
| Other Notes: | To gain a comprehensive understanding of our extensive range of Lipopolysaccharides for your research, we encourage you to visit our Carbohydrates Category page. |
| Packaging: | 1 mg in glass bottle |
| Preparation Note: | Lipopolysaccharides are supplied as lyophilized, γ-irradiated powders. To reconstitute, add 1 ml sterile balanced salt solution or tissue culture medium to the vial (1 mg) and gently swirl until the powder dissolves. Reconstituted product may be further diluted to desired working concentrations using sterile balanced salt solution or tissue culture medium. |
| Symbol |  GHS06 |
| Signal word | Danger |
| Hazard statements | H300 |
| Precautionary statements | P264 - P270 - P301 + P310 - P405 - P501 |
| Hazard Codes | Xn |
| Risk Statements | 20-21-22 |
| Safety Statements | 36 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | 2-8°C |
| UNSPSC | 12352211 |