Lyticase from Arthrobacter luteus
SIGMA/L4025 - lyophilized powder, ≥200 units/mg solid
Synonym: (1,3)-β-D-Glucan endohydrolase; 1,3-β-Glucan glucohydrolase; Bacterial lyticase; Lysing enzyme
CAS Number: 37340-57-1
MDL Number: MFCD00131560
Product Type: Chemical
Lyticase specificity: Yeast β-glucan is a polymer of β-(1-3)-D-glycopyranosyl units with branching at β-(1-6)-D-glycopyranosyl units.
Yeast cells are difficult to disrupt because the cell walls may form capsules or resistant spores. DNA can be extracted from yeast by using lysing enzymes. Lyticase is preferred to digest cell walls of yeast and generate spheroplasts from fungi for transformation. Lyticase contains β-(1→3)-glucan laminaripentaohydrolase with additional β-(1→3)-glucanase, protease, and mannanase activities.
| application(s) | diagnostic assay manufacturing |
| biological source | bacterial (Arthrobacter luteus) |
| form | lyophilized powder |
| Quality Level | 200  |
| specific activity | ≥200 units/mg solid |
| storage temp. | −20°C |
| suitability | suitable for cell lysis |
| technique(s) | cell based assay: suitable |
| Application: | Lyticase from Arthrobacter luteus has been used for resuspending cells to extract high-molecular weight DNA for polymerase chain reaction (PCR) fingerprint analyses. |
| Application: | Lyticase from Arthrobacter luteus has been used: • as a component in spheroplasting buffer to prepare yeast nuclear extracts • for digestion in sample preparation for β-glucan enzymatic assay using yeast • to lyse cells for RNA isolation |
| Biochem/physiol Actions: | Lyticase enzyme is frequently used in fungal research, particularly for species identification using polymerase chain reaction (PCR)-based techniques. It can break down β (1 |
| Biochem/physiol Actions: | Lyticase hydrolyzes poly-β(1→3)-glucose such as yeast cell wall glucan. |
| Biochem/physiol Actions: | Lyticase is a lysing enzyme that is used to extract DNA from yeast cells by inducing partial spheroplast formation. Spheroplasts are subsequently lysed to release DNA. Lyticase is preferred to digest cell walls of yeast, which are difficult to disrupt because the cell walls may form capsules or resistant spores. Lyticase contains β-(1 |
| Other Notes: | For R&D use only. Not for drug, household, or other uses. Please consult the Safety Data Sheet for information regarding hazards and safe handling practices. |
| Other Notes: | One unit will produce a ΔA800 of 0.001 per min at pH 7.5 at 25 °C, using a suspension of yeast as substrate in a 3 mL reaction mixture. |
| Other Notes: | View more information on enzymes for complex carbohydrate analysis at www.sigma-aldrich.com/enz |
| Packaging: | 25000, 50000, 100000, 250000, 1000000 units in poly bottle |
| Symbol |  GHS08 |
| Signal word | Danger |
| Hazard statements | H334 |
| Precautionary statements | P261 - P284 - P501 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| activity | specific activity: ≥200 units/mg solid |
| Storage Temp. | −20°C |
| UNSPSC | 12352204 |