Lectin from Triticum vulgaris (wheat)

SIGMA/L3892 - peroxidase conjugate, lyophilized powder

Synonym: WGA; Wheat germ agglutinin

Product Type: Chemical

Catalog Number	PKG Qty.	Price


45-L3892-1MG	1 mg	$281.00 1/EA	Add To Favorites
45-L3892-2MG	2 mg	$510.00 1/EA	Add To Favorites
45-L3892-5MG	5 mg	$962.00 1/EA	Add To Favorites

Western Blot of twelve glycoproteins using HRP conjugated Triticum vulgaris lectin specific for GlcNAc)2, NeuNAc.
Lanes
1. Urokinase from human urine (Sigma U0633) (glcNAc,Fuc)
2. Factor IX Human (Sigma F0806) (galNAc, Glc, glcNAc, Fuc)
3. Complement component C1q from human serum (Sigma C1740) (galNAc, Gal)
4. a1-Antitrypsin from human plasma (Sigma A3106) (glcNac)
5. Fetuin (Sigma F2379) (glcNac)
6. Invertase glycoprotein Standard (Sigma I0408) (glcNac)
7. IgG1 Monoclonal Anti-Flag M2 Ab produced in mouse (Sigma F3165) (Gal, Man, glcNac, Fuc)
8. IgG2a Monoclonal Anti-AMY1A Ab produced in mouse (Sigma WH0000276M4) (Gal, Man, glcNac, Fuc)
9. Recombinant Human t-Plasminogen Activator/tPA (R&D Systems 7449-SE-010) (glcNAc, Fuc)
10. Epidermal growth factor receptor human (Sigma E3641) (glcNAc)
11. Apolipoprotein C-III from human plasma (Sigma A3106)
12. Thrombospondin from human platelets (Calbiochem 605225)

Agglutination
assay: Lectin from Triticum vulgaris (wheat) (Cat. No.
L3892) at a concentration of ~1mg solid/mL was serially diluted (2-fold, top
to bottom of plate) and incubated with human erythrocytes type: A (4% v/v
RBC) to calculate Minimum agglutination titer (MAT). Agglutination is defined
as the inability of cells to form a tight button. PBS was used as blank.

Properties
Descriptions
Safety Info.
Basic Data

assay	≥85% protein basis (Warburg-Christian)
biological source	Triticum vulgaris
composition	Protein, ~90% modified Warburg-Christian
conjugate	peroxidase conjugate
form	lyophilized powder
peroxidase activity	50-200 units/mg protein
potency	<40 μg/mL agglutination activity
Quality Level	200
storage temp.	−20°C

Application:	Detects glycoproteins containing β(1→4)-N-acetyl-D-glucosamine when used with appropriate peroxidase substrate
Application:	General Western Blot Protocol: • Glycoprotein sample size: 500ng • Lectin Concentration: 0.1ug/ml Load samples at 500 ng of glycoprotein per lane • Run 4-20% Bis-Tris SDS page gel • Transfer gel to a PVDF membrane • Block membrane for 1 hr at RT with RIPA buffer (R0278 Sigma) • Incubate HRP lectin at 0.1ug/ml with RIPA buffer for 2 hours at RT • Wash membrane 5 x 5 minutes with 25ml RIPA buffer • Detect using chemiluminescent substrate (CPS1-120)
Biochem/physiol Actions:	WGA is not blood group specific but has an affinity for N-acetyl-β-D-glucosaminyl residues and N-acetyl-β-D-glucosamine oligomers. WGA contains no protein-bound carbohydrate.
Other Notes:	One unit will form 1.0 mg of purpurogallin from pyrogallol in 20 sec at pH 6.0 at 20 °C.
Packaging:	1 mg in glass bottle
Packaging:	2 mg in serum bottle
Packaging:	Package size based on protein content.
Physical form:	Contains citrate buffer
Preparation Note:	Conjugates are prepared from affinity purified lectin.
Preparation Note:	Prepared from peroxidase type VI (P8375) using a modification of the method of O′Sullivan, which favors low molecular weight conjugates. Repurified after conjugation by affinity chromatography.

RIDADR	NONH for all modes of transport
WGK Germany	WGK 3
Flash Point(F)	Not applicable
Flash Point(C)	Not applicable

Purity	≥85% protein basis (Warburg-Christian)
activity	peroxidase activity: 50-200 units/mg protein
Storage Temp.	−20°C
UNSPSC	12352202

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