D-Lactic Dehydrogenase from Lactobacillus leichmannii
SIGMA/L3888 - lyophilized powder, 150-500 units/mg protein
Synonym: (R)-Lactate:NAD+ oxidoreductase; D-LDH
CAS Number: 9028-36-8
EC Number: 232-829-0
MDL Number: MFCD00130907
Product Type: Chemical
Unit Definition One unit will reduce 1.0 μmole of pyruvate to D-lactate per min at pH 7.0 at 25 °C.
| biological source | bacterial (Lactobacillus leichmannii) |
| composition | Protein, ~50% biuret |
| foreign activity | Malic dehydrogenase <0.5% of base activity |
| form | lyophilized powder |
| Quality Level | 200  |
| specific activity | 150-500 units/mg protein |
| storage temp. | −20°C |
| Application: | D-Lactic Dehydrogenase from Lactobacillus leichmannii has been used: • in lactate dehydrogenase activity for testing the chaperone activity of proteins • to test the kinase activities of purified thiamine monophosphate(ThiM) • in NADH-coupled steady-state ATPase assay • to determine cellular lactate |
| Application: | In the food industry, the primary catalysis is coupled to conversion of NADH and H+ to NAD+ with diaphorase coupled with converting the non-fluorescent resazurin to the highly fluorescent substance resorufin to measure the content of |
| Biochem/physiol Actions: | |
| Biochem/physiol Actions: | It acts as a crucial checkpoint in gluconeogenesis and DNA metabolism. Elevated levels of LDH in the blood have been observed in various conditions, including heart attacks, cancers, liver disease, muscle trauma, anemia, bone fractures, and infections such as encephalitis, human immunodeficiency virus(HIV), and meningitis. LDH also serves as a non-specific marker of tissue turnover, which is a normal metabolic process. Additionally, reduced D-LDH activity has been found in case of mutations in LDHD found in patients with D-lactic acidosis. |
| General description: | Research area: Cell Signaling Lactate Dehydrogenase (LDH) is classified as an oxidoreductase and is found in various organisms, including both plants and animals. LDH is widely distributed across all tissues, with high concentrations in muscle, kidney, and liver. The genes encoding LDH are LDHA, LDHB, LDHC, and LDHD. The D-isomer is produced by LDHD. There are two types of D-LDHs: NAD-dependent D-LDHs and FAD-dependent D-LDHs. |
| Other Notes: | |
| Other Notes: | One unit will reduce 1.0 μmole of pyruvate to |
| Packaging: | 500, 2500 units in poly bottle |
| Physical form: | Lyophilized powder containing phosphate buffer salts |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| activity | specific activity: 150-500 units/mg protein |
| Storage Temp. | −20°C |
| Enzyme Commission (EC) Number | 1.1.1.28 ( BRENDA  | IUBMB ) |
| UNSPSC | 12352204 |