Lysozyme from chicken egg white

SIGMA/L3790 - 10 mg/mL

Synonym: Mucopeptide N-acetylmuramoylhydrolase; Muramidase

CAS Number: 12650-88-3
MDL Number: MFCD00131557
Product Type: Chemical

Catalog Number	PKG Qty.	Price


45-L3790-10X1ML	1 mL	$144.00 10/EA	Add To Favorites

Lysozyme specificity: Peptidoglycans are polymers of β-(1-4)-N-Acetyl-D-glucosamine units. Alternating residues are modified to form N-acetylmuramic acid with the addition of lactate to form branching links to the tetrapeptide.

Properties
Descriptions
Safety Info.
Basic Data

application(s)	cell analysis
biological source	chicken egg white
concentration	≥400000 units/mL
	10 mg/mL
form	liquid
mol wt	single-chain 14.3 kDa
Quality Level	200
shipped in	wet ice
storage temp.	−20°C
suitability	suitable for cell lysis
technique(s)	cell based assay: suitable
UniProt accession no.	P00698

Application:	Enzyme breaks down the cell walls of bacteria; used to prepare spheroplasts.
Application:	Suitable for the purification of both DNA and protein from bacteria.
Application:	Tested for suitability with CelLytic^™ B in protein extraction from bacteria.
Biochem/physiol Actions:	Lysozyme hydrolyzes β(1→4) linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues in peptidoglycan and between N-acetyl-D-glucosamine residues in chitodextrin. Gram-positive cells are quite susceptible to this hydrolysis as their cell walls have a high proportion of peptidoglycan.
Biochem/physiol Actions:	Lysozyme hydrolyzes β(1→4) linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues in peptidoglycan and between N-acetyl-D-glucosamine residues in chitodextrin. Gram-positive cells are quite susceptible to this hydrolysis as their cell walls have a high proportion of peptidoglycan. Gram-negative bacteria are less susceptible due to the presence of an outer membrane and a lower proportion of peptidoglycan. However, these cells may be hydrolyzed in the presence of EDTA that chelates metal ions in the outer bacterial membrane. The enzyme is active over a broad pH range (6.0 to 9.0). At pH 6.2, maximal activity is observed over a wider range of ionic strengths (0.02 to 0.100 M) than at pH 9.2 (0.01 to 0.06 M).
General description:	Lysosyme is an enzyme found in egg white that breaks down the cell walls in bacteria enhancing the efficiency of DNA and protein extraction.
Legal Information:	CelLytic is a trademark of Sigma-Aldrich Co. LLC
Other Notes:	One unit will lyse 0.4 μg of Micrococcus lysodeikticus per minute by turbidimetric detection at 600 nm when suspended in buffer at pH 8.0 at 37 °C.
Physical form:	Ready to use solution:10 mg/mL lysozyme in 25 mM sodium acetate pH 4.5 with 50% glycerol.
Physical form:	Solution in 50% glycerol, 25 mM Sodium Acetate

Symbol	GHS08
Signal word	Danger
Hazard statements	H334
Precautionary statements	P261 - P284 - P501
RIDADR	NONH for all modes of transport
WGK Germany	WGK 2
Flash Point(F)	Not applicable
Flash Point(C)	Not applicable

Storage Temp.	−20°C
Enzyme Commission (EC) Number	3.2.1.17 ( BRENDA \| IUBMB )
UNSPSC	12352204

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