LB Broth (Miller)
SIGMA/L3522 - Highly-referenced nutrient-rich microbial growth powder medium, suitable for regular E.coli culture
Synonym: Luria Broth; Miller’s LB Broth
Product Type: Chemical
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| application(s) | food and beverages |
| composition | NaCl, 10 g/L |
| Tryptone, 10 g/L | |
| Yeast Extract, 5 g/L | |
| description | Miller′s Modification, 10 g/L NaCl |
| form | powder |
| grade | Molecular Biology |
| pH | 6.8-7.2(2.5% solution) |
| Quality Level | 200  |
| sterility | non-sterile |
| storage temp. | room temp |
| suitability | nonselective for Escherichia coli |
| nonselective for coliforms |
| Application: | LB Broth (Miller) has been used as a culture medium for E coli, Pseudomonas putida KT2440, Vibrio parahaemolyticus, and soil samples for the isolation of Bacillus spp. Suitable for non-selective cultivation of E. coli strains for cloning, DNA plasmid production and production of recombinant proteins. Also suitable for selective cultivation when appropriate antibiotics are added. |
| Biochem/physiol Actions: | Luria Broth is used for maintenance and propagation of Escherichia coli. The E. coli grow faster in Luria Broth because the tryptone and yeast supply essential growth factors that the E. coli would otherwise have to synthesize. Luria Broth also contains essential electrolytes for transport and osmotic balance, due to the NaCl component. |
| Features and Benefits: | Miller’s LB powder provides: • Easy scale-up using larger package sizes • A budget-friendly alternative to liquid • Standard formulation |
| General description: | Miller’s LB is a highly-referenced microbial growth medium used for the cultivation of E. coli. This nutrient-rich microbial broth contains peptides, amino acids, water-soluble vitamins, and carbohydrates. |
| Preparation Note: | 1. Suspend 25 g in 1 L of distilled water. 2. Autoclave for 15 minutes at 121 °C. To prepare the medium of Luria, Adams and Ting (also known as LC broth: Aseptically add 25 ml of sterile 0.1 M calcium chloride after autoclaving. |
| Preparation Note: | Stir to suspend 25g powder in 1L water. Autoclave for 15 minutes at 121C to sterilize. Allow to cool before making additions, such as antibiotics (if desired). |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | room temp |
| UNSPSC | 41106200 |