LB Broth (Luria low salt)
SIGMA/L3397 - Powder microbial growth medium
Synonym: Luria Broth (Miller’s Modification); Luria-Bertani medium (LB)
Product Type: Chemical
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: L3397-250G
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: L3397-1KG
| application(s) | food and beverages microbiology |
| composition | NaCl, 0.5 g/L |
| Tryptone, 10 g/L | |
| Yeast Extract, 5 g/L | |
| form | powder |
| grade | Molecular Biology |
| pH | 6.8-7.2(1.55% solution) |
| Quality Level | 200  |
| sterility | non-sterile |
| storage temp. | room temp |
| suitability | nonselective for Escherichia coli |
| nonselective for coliforms | |
| suitable for molecular biology | |
| technique(s) | microbiological culture: suitable |
| Application: | LB Broth (Luria low salt) has been used as a liquid broth medium in the broth microdilution technique. |
| Features and Benefits: | Luria’s low salt LB powder provides: • Standard formulation • A budget-friendly alternative to liquid • Easy scale-up using larger package sizes |
| General description: | Luria Broth is a bacterial culture medium used for the cultivation of members of the Enterobacteriaceae especially E.coli, as well as for coliphage plaque assays. This nutrient-rich microbial broth contains peptides, amino acids, water-soluble vitamins, and carbohydrates in a very low-salt formulation. |
| Preparation Note: | 1. Suspend 15.5 g in 1 L of distilled water. 2. Autoclave for 15 minutes at 121°C. |
| Preparation Note: | Stir to suspend 15.5g powder in 1L water. Autoclave for 15 minutes at 121C to sterilize. Allow to cool before making additions, such as antibiotics (if desired). |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | room temp |
| UNSPSC | 41106200 |