LB Broth with agar (Lennox)
SIGMA/L2897 - Highly-referenced microbial growth powder medium with Agar, low salt, suitable for salt-sensitive E. coli culture.
Synonym: Lennox broth; Luria Bertani Agar
Product Type: Chemical
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: L2897-250G
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: L2897-1KG
| application(s) | food and beverages microbiology |
| composition | Agar, 15 g/L |
| NaCl, 5 g/L | |
| Tryptone, 10 g/L | |
| Yeast Extract, 5 g/L | |
| form | powder |
| grade | Molecular Biology |
| Quality Level | 200  |
| sterility | non-sterile |
| storage temp. | room temp |
| suitability | nonselective for Escherichia coli |
| nonselective for coliforms | |
| technique(s) | microbiological culture: suitable |
| Application: | LB Broth with agar (Lennox) has been used for: • the transformation of competent E. coli cells • to cultivate Ecc-15 bacteria • embedding scaffold containing tissue derived from induced pluripotent stem cells (iPSCs) • transmission electron microscopy (TEM) and immunohistochemistry (IHC) It is suitable for non-selective cultivation of E. coli strains for cloning, DNA plasmid production and production of recombinant proteins. Also suitable for selective cultivation when appropriate antibiotics are added, including those that require low-salt conditions, such as Zeocin®. |
| Features and Benefits: | Lennox LB powder with agar provides: • A budget-friendly alternative to pre-poured plates • Easy scale-up using larger package sizes • Standard formulation |
| General description: | Lennox LB is a highly-referenced microbial growth medium used for the cultivation of E. coli. This nutrient-rich microbial broth contains peptides, amino acids and carbohydrates in a low-salt formulation. |
| Legal Information: | Zeocin is a registered trademark of Cayla Sarl |
| Preparation Note: | 1. Suspend 35 g in 1 L of distilled water. 2. Heat to boiling while stirring to dissolve all ingredients completely. 3. Autoclave for 15 minutes at 121°C. To prepare Lennox L Agar: Add 1 g glucose and proceed with preparation instructions as above.To prepare the medium of Enquist and Sternberg: Aseptically add 10 ml sterile 1 M magnesium sulfate after autoclaving. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | room temp |
| UNSPSC | 41106200 |