Lipopolysaccharides from Escherichia coli O55:B5
SIGMA/L2880 - purified by phenol extraction
Synonym: LPS
CAS Number: 93572-42-0
EC Number: 297-473-0
MDL Number: MFCD00164401
Product Type: Chemical
Lipopolysaccharides (LPS) are localized in the outer layer of the membrane and are, in noncapsulated strains, exposed on the cell surface. They contribute to the integrity of the outer membrane, and protect the cell against the action of bile salts and lipophilic antibiotics.
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: L2880-100MG
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: L2880-25MG
| application(s) | cell analysis clinical research life science and biopharma |
| biological source | Escherichia coli (O55:B5) |
| color | off-white to tan |
| form | lyophilized powder |
| impurities | <3% Protein (Lowry) |
| purified by | phenol extraction |
| Quality Level | 300  |
| shipped in | ambient |
| solubility | water: soluble |
| storage temp. | 2-8°C |
| technique(s) | cell based assay: suitable |
| Application: | Lipopolysaccharide from Escherichia coli O55:B5 has been used:• to stimulate hepatocytes and non-parenchymal cells • to stimulate the spleen and study the expression of the Malitaf gene in the fish blunt snout bream • as part of the concanavalin A (ConA) and D-galactosamine/lipopolys • to check the cell viability of human chorionic villi-derived mesenchymal stem cells (hCMSCs) by methyl thiazolyl tetrazolium (MTT) assay • to induce oligodendrocyte toxicity to evaluate the neuroprotective effects of few compounds • to induce sarcopenia in rats to evaluate the antioxidant effects of curcumin-loaded hydrophobic surface-modified hydroxyapatite |
| Application: | Lipopolysaccharides (LPSs) are characteristic components of the cell wall of Gram-negative bacteria. LPS and its lipid A moiety stimulate cells of the innate immune system by the Toll-like receptor 4 (TLR4), a member of the Toll-like receptor protein family, which recognizes common pathogen-associated molecular-patterns (PAMPs). |
| Biochem/physiol Actions: | Lipopolysaccharides (LPS) sets up a protective permeability barrier that blocks the entry of antibiotics and bile salts, which make Gram-negative bacteria innately resistant. LPS is involved in bacterial pathogenicity as the host immune system primarily encounters the LPS component of the bacteria. LPS is an endotoxin and a highly immunogenic antigen that can enhance the immune system. The lipid A of LPS stimulates host cells via toll-like receptors 4 (TLR4). |
| General description: | Lipopolysaccharides (LPS) are glycolipids, a combination of carbohydrate units linked with single or multiple fatty acids that are found in the cell wall of Gram-negative bacteria. The LPS is an essential component of the outer membrane and the LPS structure is composed of lipid A, a glucosamine-based phospholipid, a short-core oligosaccharide, and an O-antigen, a distal polysaccharide. |
| Other Notes: | 2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research  |
| Other Notes: | For additional information on our range of Biochemicals , please complete this form . |
| Other Notes: | For optimal reconstitution of LPS products, an intensive vortex is required before use, regardless of the container type. Ensure that the storage solution concentration is at least 1 mg/ml. While borosilicate (silanized) containers are recommended, polypropylene (PP) plastic containers may also be used. |
| Other Notes: | To gain a comprehensive understanding of our extensive range of Lipopolysaccharides for your research, we encourage you to visit our Carbohydrates Category page. |
| Packaging: | 10 mg in poly bottle |
| Packaging: | 25, 100, 500 mg in glass bottle |
| Symbol |  GHS06 |
| Signal word | Danger |
| Hazard statements | H300 |
| Precautionary statements | P264 - P270 - P301 + P310 - P405 - P501 |
| Hazard Codes | Xn |
| Risk Statements | 20-21-22 |
| Safety Statements | 36 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | 2-8°C |
| UNSPSC | 12352201 |