L-Lactic Dehydrogenase from rabbit muscle
SIGMA/L1254 - Type XI, lyophilized powder, 600-1,200 units/mg protein
Synonym: Anaerobic Lactate Dehydrogenase; Lactate; NAD-lactate dehydrogenase; (S)-Lactate: NAD+ oxidoreductase; L-LDH; LAD; LD
CAS Number: 9001-60-9
EC Number: 232-617-8
MDL Number: MFCD00131466
Product Type: Chemical
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: L1254-100KU
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: L1254-25KU
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: L1254-50KU
| biological source | rabbit muscle |
| color | white |
| composition | protein, 90-100% |
| foreign activity | pyruvate kinase, myokinase, malic dehydrogenase, glutamic-pyruvic transaminase, glutamic-oxalacetic transaminase and α-glycerophosphate dehydrogenase ≤0.01% |
| form | lyophilized powder |
| mol wt | 140 kDa |
| Quality Level | 200  |
| specific activity | 600-1,200 units/mg protein |
| storage condition | (Keep container tightly closed in a dry and well-ventilated place) |
| storage temp. | −20°C |
| technique(s) | activity assay: suitable |
| type | Type XI |
| Analysis Note: | Protein determined by biuret. |
| Application: | • as a component of activation and relaxing solution in ATPase activity and isometric steady-state tension measurements with muscle fiber • in Trypanosoma congolense pyruvate kinase activity assay • in pyruvate kinase (PK) assay with rice plastidic PK enzyme OsPK2 |
| Biochem/physiol Actions: | Also catalyzes the oxidation of other |
| Biochem/physiol Actions: | Muscle-type Lactic Dehydrogenase (LDH) participates in metabolic pathways and its activity is essential for anaerobic glycolysis. LDH activity is inhibited by ascorbate. LDH regenerates nicotinamide adenine dinucleotide (NAD+) from NADH and is industrially useful in poly(lactic acid) production. In the absence of oxygen, LDH participates in a fermentation reaction, catalyzes pyruvate into lactic acid, and oxidizes nicotinamide adenine dinucleotide (NADH) to NAD+. Therefore, LDH mediates the production of NAD+ essential anaerobic glycolysis pathway. Through this LDH helps maintain the physiological and biochemical functions of the cell in the absence of oxygen. |
| General description: | Research area: Cell Signaling |
| General description: | Lactic Dehydrogenase (LDH) has a total molecular weight of 140 kDa and is composed of 4 subunits which are designated M subunit (muscle) and H subunit (heart). These subunits may be mixed in any of 5 combinations (M4, M3H1, M2H2, MH3, and H4). Skeletal muscle contains LDH that is predominately M4 with some small amounts of M3H and traces of H2H2. The H and M subunits are quite similar in molecular weight, but differ substantially in amino acid composition. Rabbit muscle LDH dissociates into dimeric species (MW = ~70 kDa) in acetate-chloride at pH 5.0, the dissociation is reversible. Biochemistry, 13, 3527-3531 (1974). Oxidizes glyoxylate and lactate. Isoelectric point: 8.4-8.6 Optimal pH : 7.5 . |
| Other Notes: | One unit will reduce 1.0 μmole of pyruvate to |
| Packaging: | 1000, 5000 units in glass bottle |
| Packaging: | 25000, 50000, 100000 units in poly bottle |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| activity | specific activity: 600-1,200 units/mg protein |
| Storage Temp. | −20°C |
| Enzyme Commission (EC) Number | 1.1.1.27 ( BRENDA  | IUBMB ) |
| UNSPSC | 12352204 |