L-Lactic Dehydrogenase from bovine heart
SIGMA/L1006 - Type XVII, buffered aqueous glycerol solution, ≥400 units/mg protein
Synonym: Lactate; (S)-Lactate: NAD+ oxidoreductase; L-LDH; LAD; LD
CAS Number: 9001-60-9
EC Number: 232-617-8
MDL Number: MFCD00131466
Product Type: Chemical
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| application(s) | life science and biopharma |
| assay | 5.0—15.0 mg protein/mL (biuret) |
| biological source | bovine heart |
| cation traces | NH4+: ≤10 μg/mg protein |
| color | white |
| foreign activity | glutamic-pyruvic transaminase and glutamic-oxalacetic transaminase ≤0.02% |
| pyruvate kinase, myokinase and α-glycerophosphate dehydrogenase ≤0.03% | |
| form | buffered aqueous glycerol solution |
| impurities | <10 μg/mg protein free ammonium |
| manufacturer/tradename | Sigma-Aldrich |
| mol wt | 140 kDa |
| pH | 7.5 |
| Quality Level | 200  |
| specific activity | ≥400 units/mg protein |
| storage temp. | 2-8°C |
| technique(s) | cell based assay: suitable |
| type | Type XVII |
| UniProt accession no. | P19858  |
| Q5E9B1 |
| Analysis Note: | Protein determined by biuret |
| Application: | |
| Biochem/physiol Actions: | Also catalyzes the oxidation of other |
| Biochem/physiol Actions: | Variation in the levels of |
| General description: | Research area: Cell signaling. L-LacticDehydrogenase (LDH) is present in the cell cytoplasm. and is part of the glycolytic pathway. Lactate dehydrogenase (LDH) is a ubiquitous molecule found in plants, yeast, mammals, and microorganisms and is a member of the oxidoreductase family. It has five isozymes (LD1 to LD5) and this composition varies in different tissues. LD1 is present at higher concentrations in the heart, kidneys, and erythrocytes. LD5 is found in the liver and skeletal muscles.(2) |
| Other Notes: | One unit will reduce 1.0 μmole of pyruvate to |
| Packaging: | 12500 units in glass bottle |
| Physical form: | Solution in 50% glycerol containing 0.025 M potassium phosphate buffer, pH 7.5 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | 5.0—15.0 mg protein/mL (biuret) |
| activity | specific activity: ≥400 units/mg protein |
| Storage Temp. | 2-8°C |
| Enzyme Commission (EC) Number | 1.1.1.27 ( BRENDA | IUBMB ) |
| UNSPSC | 12352204 |