Lectin from Arachis hypogaea (peanut)
SIGMA/L0881 - lyophilized powder, Affinity-purified
Synonym: PNA; Peanut agglutinin
Product Type: Chemical
Agglutination assay: Lectin from Arachis hypogaea (peanut) (Cat. No. L0881) at a
concentration of ~1mg solid/mL was serially diluted (2-fold, top to bottom of
plate) and incubated with human erythrocytes type: O (4% v/v RBC) to
calculate Minimum agglutination titer (MAT). Agglutination is defined as the
inability of cells to form a tight button. PBS was used as blank.
concentration of ~1mg solid/mL was serially diluted (2-fold, top to bottom of
plate) and incubated with human erythrocytes type: O (4% v/v RBC) to
calculate Minimum agglutination titer (MAT). Agglutination is defined as the
inability of cells to form a tight button. PBS was used as blank.
SDS-PAGE Lectin from Arachis hypogaea (peanut) (Cat. No. L0881) was assessed on SDS-PAGE.
| form | lyophilized powder |
| impurities | salt, free |
| potency | <0.1 μg per mL agglutination activity |
| quality | Affinity-purified |
| Quality Level | 200  |
| storage temp. | −20°C |
| Analysis Note: | Agglutination activity is expressed in μg/ml and is determined from serial dilutions in phosphate buffered saline, pH 6.8, of a 1 mg/ml solution. This activity is the lowest concentration to agglutinate a 2% suspension of neuraminidase treated human blood group O erythrocytes after 1 hr incubation at 25 °C. |
| Application: | Lectin from Arachis hypogaea (peanut) has been used: • to detect Thomson-Friedenreich antigen • as a component in PBS to study its effects on cell viability • to study its ability to block the binding site |
| Biochem/physiol Actions: | Lectin from Arachis hypogaea (peanut) possess specificity towards β - |
| Biochem/physiol Actions: | PNA does not agglutinate normal human erythrocytes, but strongly agglutinates neuraminidase treated erythrocytes. PNA has potent anti-T activity similar to the anti-T antibody in human sera. The lectin can be used to distinguish between human lymphocyte subsets. |
| General description: | Lectin from Arachis hypogaea (peanut) is a homotetrameric protein of 110 kDa. |
| Packaging: | 5, 10, 25 mg in glass bottle |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352202 |