Lipase from Aspergillus oryzae
SIGMA/L0777 - solution, ≥100,000 U/g, white, beige
Synonym: AOL; Lipolase 100L
CAS Number: 9001-62-1
EC Number: 232-619-9
MDL Number: MFCD00131509
Product Type: Chemical
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| biological source | Aspergillus sp. (Aspergillus oryzae) |
| color | beige |
| white | |
| form | solution |
| InChI | 1S/C11H9N3O2.Na/c15-8-4-5 |
| InChI key | QWZUIMCIEOCSJF-CHHCPSLASA |
| Quality Level | 200  |
| specific activity | ≥100,000 U/g |
| storage condition | (Tightly closed. Dry) |
| storage temp. | 2-8°C |
| technique(s) | cell based assay: suitable |
| UniProt accession no. | I8ABR3  |
| I8ADK3 |
| Application: | Lipase from Aspergillus oryzae has been used: • as a control enzyme in activity assays • as a component in lipolase stock solution for the hydrolysis experiments • to incubate scaffolds for lipase-accelerated degradation experiments • as the lipolytic enzyme standard to detect lipolytic enzymatic activity via chromogenic agar plates and zymography |
| Biochem/physiol Actions: | Lipase is widely used in several industries including food and pharmaceuticals. It mediates the hydrolysis of fats and oil. Lipase is utilized for peptide synthesis and in the detergent industry. It is active in the range of pH 2-5 and temperature between 30-50°C. Metal ions such as Fe2+, Fe3+, and Cu2+ prevent the action of lipase. A less polar organic solvent is preferred for high stability.Lipases play a crucial role in digestion as well as the transportation and processing of dietary lipid substrates by catalyzing the hydrolysis of ester bonds in lipid substrates. The lipase from Aspergillus oryzae (AOL), a multipurpose biocatalyst can be used in the kinetic resolution of a biotin intermediate lactone,α-lipoic acid, and 1-phenylethanol. Additionally, it has the ability to stereoselectively catalyze the hydrolysis of ethyl 2-(4-hydroxyphenoxy) propanoate and its analogs, which are key intermediates in the production of aryloxyphenoxypropionate herbicides. AOL serves as a promising biocatalyst in the esterification of a series of short-chain acids and alcohols to produce flavor esters. It can be used to catalyze the esterification of lauric acid with aromatic alcohol-benzyl alcohol. |
| General description: | Research area: Cell Signaling Lipase is derived from Aspergillus oryzae by ammonium sulfate precipitation and chromatography.Lipases or triacylglycerol acyl hydrolases are a group of hydrolase enzymes that are usually found in humans and animals with monogastric stomachs. Lipase enzymes are generally formed in the pancreas and stomach where they aid in fat and lipids digestion.(2) |
| Legal Information: | A product of Novozyme Corp. |
| Packaging: | 50 mL in glass bottle |
| Preparation Note: | Produced by submerged fermentation of a genetically modified Aspergillus oryzae microorganism |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| activity | specific activity: ≥100,000 U/g |
| Storage Temp. | 2-8°C |
| Enzyme Commission (EC) Number | 3.1.1.3. |
| UNSPSC | 12352204 |