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Lectin from Pisum sativum (pea)

SIGMA/L0770 - FITC conjugate, lyophilized powder

Synonym: Pisum sativum agglutinin; PSA

Product Type: Chemical
Catalog Number PKG Qty. Price Quantity
45-L0770-2MG 2 mg
 $185.00
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Agglutination assay: Lectin from Pisum sativum (pea) (Cat. No. L0770) was serially diluted (2-fold, top to bottom of plate) and incubated with human erythrocytes type: O (4% v/v RBC) to calculate Minimum agglutination titer (MAT). Agglutination is defined as the inability of cells to form a tight button. PBS was used as blank.

 

composition Protein, ≥95% biuret
conjugate FITC conjugate
extent of labeling 2-4 mol FITC per mol protein (Lowry).
form lyophilized powder
impurities salt, free
potency <20 μg per mL agglutination activity
Quality Level 200 
storage temp. −20°C
Analysis Note: Agglutination activity is expressed in μg/ml and is determined from serial dilutions in phosphate buffered saline, pH 6.8, of a 1 mg/ml solution. This activity is the lowest concentration to agglutinate a 2% suspension of human erythrocytes after 1 hr incubation at 25 °C.
Application: Lectin from Pisum sativum (pea) has been used to determine acrosomal status and viability of spermatozoa and to assess sperm acrosome reaction (AR) patterns
Biochem/physiol Actions: Lectin from Pisum sativum (pea) possess anti-hepatoma effect and mitogenicity to lymphocytes, like concanavalin A (Con A).
Biochem/physiol Actions: PSA is not blood group specific, but has an affinity for terminal α-D-mannosyl residues. PSA is a mitogen similar to concanavalin A.
General description: Lectin from Pisum sativum (pea) is a tetrameric glycoprotein of about 48 kDa. It is isolated from peas. Pisum sativum agglutinin is specific for α-D-glucose and α-D-mannose.
Packaging: 2 mg in serum bottle
Preparation Note: Conjugates are prepared from affinity purified lectin.
Symbol GHS08  GHS08
Signal word Danger
Hazard statements H317 - H334
Precautionary statements P261 - P280 - P342 + P311
Hazard Codes Xn
Risk Statements 42/43
Safety Statements 36
RIDADR NONH for all modes of transport
WGK Germany WGK 3
Flash Point(F) Not applicable
Flash Point(C) Not applicable
Storage Temp. −20°C
UNSPSC 12352202

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