Anti-LAMP2 antibody produced in rabbit
SIGMA/L0668 - affinity isolated antibody, buffered aqueous solution
Synonym: Anti-CD107B; Anti-LGP110; Anti-Lysosomal Membrane Glycoprotein-type B (LGP-B); Anti-
MDL Number: MFCD01864612
Product Type: Chemical
Immunocytochemistry-immunoflourescence JOURNAL CITATION: Itm2a expression in the developing mouse first lower molar, and the subcellular localization of Itm2a in mouse dental epithelial cells. By: Kihara, M., Kiyoshima, T., et al. in PLoS One, 2014. PubMed ID: 25079563 Image collected and cropped by CiteAb from the following publication, (http://dx.plos.org/10.1371/journal.pone.0103928), provided under a CC-BY license. Image might reflect a new usage that is not reflected in claims on product description or independently verified by Merck KGaA, Darmstadt, Germany. For Research Use Only. Not for use in diagnostic procedures.
Immunofluorescence
Rat NRK cells were fixed and permeabilized with 4% paraformaldehyde followed by 0.5% Triton-X-100. Fixed cells were stained with 10 μg/mL Anti-LAMP2 (Cat. No. L0668) and developed with Goat Anti-Rabbit IgG, FITC conjugate.
Rat NRK cells were fixed and permeabilized with 4% paraformaldehyde followed by 0.5% Triton-X-100. Fixed cells were stained with 10 μg/mL Anti-LAMP2 (Cat. No. L0668) and developed with Goat Anti-Rabbit IgG, FITC conjugate.
Immunofluorescence NIH/3T3 Mouse fibroblastcells were fixed and permeabilized with 4% paraformaldehyde followed by 0.5% Triton X-100. Fixed cells were stained with 20 μg/m Anti-LAMP2 antibody produced in Rabbit (Cat. No. L0668). The antibody was developed using Anti-Rabbit IgG (whole molecule)-FITC antibody produced in Goat (Cat. No. F9887).
Western Blotting Whole extract of NRK was separated on SDS-PAGE and probed with 2.5 μg/mL Anti-Phosphotyrosine antibody, Mouse monoclonal (Cat. No. L0668). The antibody was developed using Anti-Rabbit IgG (whole molecule)-Peroxidase antibody produced in Goat (Cat. No. A0545) and chemiluminescent substrate. Lanes 1. 5 μg/mL antibody 2. 2.5 μg/mL antibody
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| mol wt | antigen ~110 kDa |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | human (predicted), mouse, rat |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | indirect immunofluorescence: 10-20 μg/mL using rat NRK and mouse NIH3T3 cells |
| western blot (chemiluminescent): 2.5-5 μg using whole extract of rat NRK cells | |
| UniProt accession no. | P13473  |
| Application: | Anti-LAMP2 antibody can be used in immunofluorescence and immunoblotting |
| Application: | Anti-LAMP2 antibody produced in rabbit has been used in immunohistochemical analysis. |
| Application: | Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below. Western Blotting (1 paper) |
| Biochem/physiol Actions: | Lysosomal associated membrane protein 2 (LAMP2) promotes cell-cell adhesion. It maintains cellular homeostasis and stress. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | LAMP2 (lysosome-associated membrane protein 2) is a heavily glycosylated intrinsic protein (110kD) present in lysosomal membrane. The protein glycosylation is essential for its function to protect the lysosomal membrane from proteolytic enzymes. Anti-LAMP2 antibody can be used in western blotting. It can also be used as a lysosomal marker. Rabbit anti-LAMP2 antibody reacts specifically with LAMP2 of mouse and rat. |
| General description: | LAMP2 encodes a member of a family of membrane glycoproteins. This glycoprotein provides selectins with carbohydrate ligands. It may play a role in tumor cell metastasis. It may also function in the protection, maintenance, and adhesion of the lysosome. |
| General description: | Lysosomal associated membrane protein 2 (LAMP2) gene is located on human chromosome Xq24. LAMP2 is majorly expressed in lysosomes. It has three isoforms, that is LAMP2A, LAMP2B and LAMP2C. |
| Immunogen: | synthetic peptide corresponding to amino acid residues 404-415 of mouse LAMP2 with N-terminal added cysteine, conjugated to KLH. The corresponding sequence is identical in rat and differs by two amino acids in human. |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |