Lipase from porcine pancreas
SIGMA/L0382 - Type VI-S, ≥20,000 units/mg protein, lyophilized powder
Synonym: PPL; Triacylglycerol acylhydrolase; Triacylglycerol lipase
CAS Number: 9001-62-1
EC Number: 232-619-9
MDL Number: MFCD00131509
Product Type: Chemical
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: L0382-1MU
| composition | Protein, 40-70% |
| form | lyophilized powder |
| InChI | 1S/C11H9N3O2.Na/c15-8-4-5 |
| InChI key | QWZUIMCIEOCSJF-CHHCPSLASA |
| Quality Level | 300  |
| shipped in | wet ice |
| specific activity | ≥20,000 units/mg protein |
| storage temp. | −20°C |
| type | Type VI-S |
| Analysis Note: | Protein determined by biuret. |
| Application: | Lipase from porcine pancreas has been used: • in lipase inhibition assay with plant extracts • in in vitro digestion gelatinized starch/fiber mixtures • with simulated intestinal fluid to mimic the digestive tract condition of by L. fermentum PC1 in fermented oats |
| Application: | Lipases are used industrially for the resolution of chiral compounds and the transesterification production of biodiesel. |
| Biochem/physiol Actions: | Lipase from porcine pancreas (PPL) catalyzes the hydrolysis of fats (lipids). It plays an essential role in the digestion, transport, and processing of dietary lipids (e.g. triglycerides, fats, oils) in most living organisms. PPL is cost-effective and is useful in biotransformation reactions. It displays high selectivity, and catalytic activity. PPL is also used in the detergent formulation for removing oil stains from clothes. |
| Biochem/physiol Actions: | Lipases catalyze the hydrolysis of esters in aqueous solutions and the synthesis of esters in non-aqueous solutions. They also produce hydroxyl and carboxylic groups through the hydrolysis of ester linkages in poly(ethylene terephthalate). Lipases catalyze the hydrolysis of triacylglycerols into glycerol and free fatty acids. |
| General description: | Lipase from porcine pancreas (PPL) or porcine pancreatic lipase is a small globular protein with N-terminal α/β type fold. It comprises catalytic triad Ser, Asp, and His residues and a C-terminal β-sandwich domain. PPL belongs to a subclass of the carboxylesterases family. |
| Other Notes: | One unit will hydrolyze 1.0 microequivalent of fatty acid from a triglyceride in 1 hr at pH 7.7 at 37 °C using olive oil. |
| Packaging: | 100000 units in glass bottle |
| Packaging: | 1000000 units in poly bottle |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| activity | specific activity: ≥20,000 units/mg protein |
| Storage Temp. | −20°C |
| Enzyme Commission (EC) Number | 3.1.1.3 ( BRENDA  | IUBMB ) |
| UNSPSC | 12352204 |