KiCqStart® One-Step Probe RT-qPCR ReadyMix™
SIGMA/KCQS07 - for Bio-Rad, Cepheid, Eppendorf, Illumina, Corbett, and Roche systems
Synonym: KiCqStart® One Step Probe RT-qPCR Readymix™
Product Type: Chemical
KiCqStart One-Step Probe RT-qPCR Readymix supports reproducible gene amplification across a wide dynamic range.
KiCqStart One-Step Probe RT-qPCR ReadyMix is highly resistant to common sample-based inhibitors.
| color | light blue |
| compatibility | Bio-Rad CFX384 |
| Bio-Rad CFX96 | |
| Bio-Rad MiniOpticon | |
| Bio-Rad MJ Chromo4 | |
| Bio-Rad MJ Opticon | |
| Bio-Rad MJ Opticon 2 | |
| Eppendorf® Mastercycler ep realplex | |
| Eppendorf® Mastercycler ep realplex2 s | |
| Illumina Eco qPCR | |
| Qiagen Corbett Rotor-Gene 3000 | |
| Qiagen Corbett Rotor-Gene 6000 | |
| Qiagen Corbett Rotor-Gene Q | |
| Roche LightCycler 480 | |
| detection method | probe-based |
| feature | dNTPs included |
| hotstart | |
| form | liquid |
| input | purified RNA |
| shipped in | dry ice |
| storage condition | protect from light |
| storage temp. | −20°C |
| technique(s) | qPCR: suitable |
| usage | sufficient for 500 reactions |
| Application: | KiCqStart® One-Step Probe RT-qPCR ReadyMix™ has been used:• in quantitative real-time polymerase chain reaction (qRT-PCR) for gene expression studies • in cell type-specific transcript analysis for determining the cycle threshold (Ct) value • for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) RNA detection from nasopharyngealand oropharyngeal swabs • for amplification of reverse-transcribed cDNA |
| Features and Benefits: | • Assay results in as little as 33 minutes • Highly efficient and sensitive real-time PCR results • Little/no optimization required |
| General description: | KiCqStart® One-Step Probe RT-qPCR ReadyMix™ is a ready-to-use, highly sensitive master mix for reverse transcription quantitative PCR (RT-qPCR) of RNA templates using hybridization probe detection chemistries such as TaqMan® 5’-hydrolysis probes on real-time PCR systems (refer to instrument compatibility section to select the appropriate reagent for your machine). First-strand cDNA synthesis and PCR amplification are carried out in the same tube without opening between procedures. It is ideal for highly sensitive quantification of RNA viruses or low abundance RNA targets as well as high throughput gene-expression studies. The system has been optimized to deliver maximum RT-PCR efficiency, sensitivity, and specificity in reduced reaction volumes and fast cycle times. KiCqStart® One-Step Probe RT-qPCR ReadyMix™ contains all required components for RT-qPCR except RNA template and probe. It is compatible with all dual-labeled probe chemistries. The reverse transcriptase is an engineered M-MLV with reduced RNase H activity and improved activity and stability at higher temperatures. The use of higher temperatures (50 to 55°C) for the first-strand step of one-step RT-qPCR provides higher specificity for primer annealing and disruption of RNA secondary structure that can interfere with cDNA synthesis. KiCqStart® One-Step Probe RT-qPCR ReadyMix™ is highly resistant to PCR inhibitors. A key component of the ReadyMix™ is an ultra-pure, highly processive, thermostable DNA polymerase that is combined with high avidity monoclonal antibodies. This provides an extremely stringent automatic hot-start that minimizes the potential for primer-dimer and other non-specific PCR artifacts. The light blue color of the inert dye simplifies reaction assembly in white, or clear, plates and helps to minimize pipetting or mixing errors. The dye does not interfere with qPCR performance or affect the stability of the product. |
| Legal Information: | Bio-Rad is a registered trademark of Bio-Rad Laboratories, Inc. |
| Legal Information: | CFX384 is a trademark of Bio-Rad Laboratories, Inc. |
| Legal Information: | CFX96 is a trademark of Bio-Rad Laboratories, Inc. |
| Legal Information: | Chromo4 is a trademark of Bio-Rad Laboratories, Inc. |
| Legal Information: | Eppendorf is a registered trademark of Eppendorf AG |
| Legal Information: | KiCqStart is a registered trademark of QIAGEN Beverly Inc. |
| Legal Information: | LightCycler is a registered trademark of Roche |
| Legal Information: | Mastercycler is a registered trademark of Eppendorf AG |
| Legal Information: | MiniOpticon is a trademark of Bio-Rad Laboratories, Inc. |
| Legal Information: | Opticon is a trademark of Bio-Rad Laboratories, Inc. |
| Legal Information: | ReadyMix is a trademark of Sigma-Aldrich Co. LLC |
| Legal Information: | Rotor-Gene is a registered trademark of Qiagen GmbH |
| Legal Information: | SmartCycler is a registered trademark of Cepheid, Inc. |
| Legal Information: | TaqMan is a registered trademark of Roche Molecular Systems, Inc. |
| Other Notes: | 2X reaction buffer containing dATP, dCTP, dGTP, dTTP, magnesium chloride, reverse transcriptase, RNase inhibitor protein, hot-start DNA polymerase, inert blue qPCR dye, and stabilizers packaging: 500 reactions* = 5 X 1 mL tubes *number of reactions based on a 20uL volume |
| Other Notes: | Different real-time PCR systems employ different strategies for normalization of fluorescent signals and correction of well-to-well optical variations. It is critical to match the appropriate qPCR reagent to your specific instrument.KiCqStart® One-Step Probe RT-qPCR ReadyMix™ does not contain an internal reference dye. Compatible instruments include: • Bio-Rad® CFX384™ • Bio-Rad CFX96™ • Bio-Rad MiniOpticon™ • Bio-Rad/MJ Chromo4™ • Bio-Rad/MJ Opticon 2 • Bio-Rad/MJ Opticon™ • Cepheid SmartCycler® • Eppendorf Mastercycler® ep realplex • Eppendorf Mastercycler ep realplex2 s • Illumina Eco qPCR • Qiagen/Corbett Rotor-Gene® 3000 • Qiagen/Corbett Rotor-Gene 6000• Qiagen/Corbett Rotor-Gene Q • Roche LightCycler® 480 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 41106300 |