Krebs-Henseleit Buffer Modified
SIGMA/K3753 - With 2000 mg/L glucose, without calcium chloride and sodium bicarbonate, powder, suitable for cell culture
Product Type: Chemical
| form | powder |
| Quality Level | 500  |
| storage temp. | 2-8°C |
| technique(s) | cell culture | mammalian: suitable |
| Application: | Krebs-Henseleit Buffer Modified has been used: • to collect the rat brains for ex vivo brain slice experiments • to measure calcium and sodium in rat pulmonary arterial endothelial cells (PAECs) • to collect the uterine and forelimb blood vessel samples from horses |
| General description: | Krebs-Henseleit Buffer Modified is a balanced salt solution (BSS) that is used in cell culture and plays a role in providing cells with water and inorganic salts for normal cell metabolism. In addition, it provides a buffering system to maintain the optimal pH of the medium and acts as an irrigating, transporting, and diluting agent to maintain the intra- and extra-cellular osmotic balance. BSS provides glucose as an energy source for cell metabolism. |
| Preparation Note: | Formulated to contain 9.6 grams of powder per liter of medium. |
| Preparation Note: | Supplement with 2.1 g/L sodium bicarbonate. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | 2-8°C |
| UNSPSC | 12352207 |