Anti-KIF17 antibody produced in rabbit
SIGMA/K3638 - affinity isolated antibody, buffered aqueous solution
Synonym: Anti-KIF17B; Anti-KIF3X; Anti-KLP-2; Anti-OSM-3
MDL Number: MFCD03791822
Product Type: Chemical
Western Blotting Mouse brain S1 extract were separated on SDS-PAGE and probed with Anti-KIF17 antibody produced in Rabbit (Cat. No. K3638). The antibody was developed using 1:10,000 Anti-Rabbit IgG (whole molecule)-Peroxidase antibody produced in Goat (Cat. No. A0545). Lanes 1. 1:1,000 antibody 2. 1:2,000 antibody 3. Negative Control
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| shipped in | dry ice |
| species reactivity | mouse, rat |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | indirect immunofluorescence: 1:100 using rat pheochromocytoma PC12 cell line differentiated with NGF |
| microarray: suitable | |
| western blot: 1:1,000 using mouse brain cytosolic fraction | |
| UniProt accession no. | Q99PW8   |
| Application: | Anti-KIF17 antibody produced in rabbit has been used in immunofluorescence and immunoblotting. |
| Biochem/physiol Actions: | The kinesin superfamily of proteins (KIFs) consists of a class of microtubule-dependent motors. They play a major role in many cellular and developmental functions. The kinesin proteins are involved in organelle transport and are primarily associated with anterograde transport of vesicles and organelles in neurons, epithelial cells, and melanosomes with bidirectional transport of mitochondria. They also mediate transport between the endoplasmic reticulum (ER) and the Golgi complex. In neurons, kinesin motors conduct vesicular transport, such as of synaptic vesicle components to axons and of neurotransmitter receptors to dendrites. KIF17 conveys (in dendrites) specific cargo vesicles that contain the glutamate receptor NR2B subunit, thus KIF17 functions as the sorting machinery for NR2B. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | KIF17 belongs to the functionally diverse subgroup of the kinesin superfamily characterized by a N-terminal motor domain. KIF17 is specifically expressed in the brain, abundantly present in the gray matter, particularly in the hippocampus and cerebral cortex, but not in the white matter such as the optic nerve. KIF17, a dendritic specific motor protein, is localized in dendrites of pyramidal neurons, but not in axons or nuclei of the cerebral cortex. |
| Immunogen: | synthetic peptide corresponding to amino acids 589-606 of mouse KIF17 conjugated to KLH. |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |