Anti-KIF3A antibody produced in rabbit
SIGMA/K3513 - IgG fraction of antiserum, buffered aqueous solution
Synonym: Anti-FLA10; Anti-KLP-20
MDL Number: MFCD03791823
Product Type: Chemical
Immunohistochemistry Anti-KIF3A Antibody: Cat. No. K3513: Immunoperoxidase staining of KIF3A in formalin fixed, paraffin-embedded rat brain at 0.1 μg/mL.
Immunohistochemistry Anti-KIF3A Antibody: Cat. No. K3513: Immunoperoxidase staining of KIF3A in formalin fixed, paraffin-embedded rat brain at 0.1 μg/mL.
Immunohistochemistry Anti-KIF3A Antibody: Cat. No. K3513: Immunoperoxidase staining of KIF3A in formalin fixed, paraffin-embedded mouse brain at 0.4 μg/mL.
Immunohistochemistry Anti-KIF3A Antibody: Cat. No. K3513: Immunoperoxidase staining of KIF3A in formalin fixed, paraffin-embedded mouse brain at 0.4 μg/mL.
Western Blotting Whole cell extract of rat pheochromocytoma PC12 cell line were separated on SDS-PAGE and probed with Anti-KIF3A antibody produced in Rabbit (Cat. No. K3513). The antibody was developed using 1:10,000 Anti-Rabbit IgG (whole molecule)-Peroxidase antibody produced in Goat (Cat. No. A0545). Lanes 1. 1:2,000 antibody 2. 1:4,000 antibody 3. Negative Control
Western Blotting Whole cell extract of Rat brain homogenate were separated on SDS-PAGE and probed with Anti-KIF3A antibody produced in Rabbit (Cat. No. K3513). The antibody was developed using 1:10,000 Anti-Rabbit IgG (whole molecule)-Peroxidase antibody produced in Goat (Cat. No. A0545). Lanes 1. 1:2,000 antibody 2. 1:4,000 antibody 3. Negative Control
| antibody form | IgG fraction of antiserum |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| packaging | antibody small pack of 25 μL |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | rat, mouse, human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunohistochemistry: suitable using suitable for IHC on rat brain perfused with 4% paraformaldehyde |
| microarray: suitable | |
| western blot: 1:2,000 using rat brain homogenate and whole cell extract of rat pheochromocytoma PC12 cell line. | |
| UniProt accession no. | Q9Y496  |
| Application: | Anti-KIF3A antibody produced in rabbit has been used in immunohistochemistry and immunofluorescence. |
| Application: | Anti-KIF3A antibody produced in rabbit has been used in: • immunoblotting • immunohistochemical detection of kinesin family member 3A (KIF3A) |
| Biochem/physiol Actions: | The gene KIF3A (kinesin family member 3A) encodes a motor subunit of kinesin-2, a protein that functions in the formation and mechanism of cilia. The chromosomal region to which the gene is mapped is linked to immune diseases, such as asthma. |
| Biochem/physiol Actions: | The kinesin superfamily (KIFs) of proteins consists of a class of microtubule-dependent motors that plays a major role in many cellular and developmental functions, including organelle transport, mitosis, meiosis, and possibly long-range signaling in neurons. KIF3 plays a crucial role during development. KIF3A-/- and KIF3B-/- mice do not survive beyond mid-gestation. Embryos lacking KIF3A and KIF3B display randomization of left-right asymmetry and numerous morphological abnormalities. The KIF3A/B heterodimer exhibits a plus end-directed microtubule sliding activity. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | KIF3A/B is abundantly expressed in neuronal tissue and ubiquitously expressed in other tissues in lower amounts. |
| General description: | The gene KIF3A (kinesin family member 3A) is mapped to human chromosome 5q31.1. The gene encodes a subunit of heterotrimeric kinesin-2, which is a canonical plus-oriented anterograde IFT (intraflagellar transport) motor. |
| Immunogen: | synthetic peptide corresponding to the C-terminus of mouse KIF3A (amino acids 682-701) conjugated to KLH. |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
| WGK Germany | WGK 2 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |