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PDK1, active, His tagged human

SIGMA/K3393 - PRECISIO® Kinase, recombinant, expressed in baculovirus infected Sf9 cells, ≥70% (SDS-PAGE), buffered aqueous glycerol solution

Synonym: PDPK1; PRO0461

Product Type: Chemical
Catalog Number PKG Qty. Price Quantity
45-K3393-10UG 10 µg
 $537.00
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assay ≥70% (SDS-PAGE)
form buffered aqueous glycerol solution
mol wt ~67 kDa
product line PRECISIO® Kinase
Quality Level 200 
recombinant expressed in baculovirus infected Sf9 cells
shipped in dry ice
specific activity 102-138 nmol/min·mg
storage temp. −70°C
UniProt accession no. Q6A1A2 
  O15530 
Biochem/physiol Actions: PDK1 (3-phosphoinositide-dependent protein kinase) is activated by the presence of PtdIns (3, 4, 5) P3 or PtdIns (3, 4) P2. PDK1 then activates protein kinase B (PKB) which, in turn, inactivates glycogen synthase kinase-3 (GSK3). The phosphorylation of other proteins by PKB and GSK3 is likely to mediate many of the intracellular actions of insulin. Thus, PDK1 plays a key role in mediating many of the actions of the second messenger(s) PtdIns (3, 4, 5) P3 and/or PtdIns (3, 4) P2. The human PDK1 is a 556-residue monomeric enzyme comprising of a catalytic domain that is most similar to the PKA, PKB and PKC subfamily of protein kinases.
Biochem/physiol Actions: PDPK1 (3-phosphoinositide dependent protein kinase 1) is a serine/threonine protein kinase which is activated upon accumulation of PIP3 (phosphatidylinositol-3,4,5-trisphosphate), a PI3K (phosphoinositide 3-kinase) product. It controls AGC (protein kinase A, G, and C) kinase members, such as AKT (v-akt murine thymoma viral oncogene homolog), p70 ribosomal S6 kinase (S6K), serum- and glucocorticoid-induced protein kinase (SGK), and protein kinase C (PKC) members. It is involved in metabolism, growth, proliferation, and survival. PDPK1 is associated with growth of angiosarcoma cells.
Legal Information: PRECISIO is a registered trademark of Merck KGaA, Darmstadt, Germany
Physical form: Supplied in 50mM NaPhosphate pH 7.0, 300 mM NaCl, 150 mM imidazole, 0.1mM PMSF, 0.2 mM DTT, 25% glycerol.
RIDADR NONH for all modes of transport
WGK Germany WGK 1
Flash Point(F) Not applicable
Flash Point(C) Not applicable
Purity ≥70% (SDS-PAGE)
activity specific activity: 102-138 nmol/min·mg
Storage Temp. −70°C
UNSPSC 12352200

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