α-Ketoglutarate Dehydrogenase from porcine heart
SIGMA/K1502 - buffered aqueous glycerol solution, 0.1-1.0 units/mg protein (Lowry)
Synonym: Multienzyme 2-oxoglutarate dehydrogenase complex
CAS Number: 9031-02-1
MDL Number: MFCD00130490
Product Type: Chemical
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| foreign activity | pyruvate dehydrogenase ≤20% |
| form | buffered aqueous glycerol solution |
| Quality Level | 200  |
| shipped in | dry ice |
| specific activity | 0.1-1.0 units/mg protein (Lowry) |
| storage temp. | −20°C |
| Analysis Note: | May contain traces of polyethylene glycol. |
| Application: | α-Ketoglutarate Dehydrogenase from the porcine heart has been used: • to study the reversal of nitration by glutathione (GSH) in peroxynitrite-treated cells • to measure its activity by Spectramax M5 microplate spectrofluorimeter using heart mitochondria • as a positive control to evaluate its activity in by Spectramax GEMINI EM fluorescence microplate reader using mice neurons |
| Biochem/physiol Actions: | α-Ketoglutarate dehydrogenase (α-KGDH) is a key enzyme of bioenergetic processes and a controlling unit of metabolic flux through the Krebs cycle or tricarboxylic acid (TCA) cycle. It catalyzes the oxidative decarboxylation of α-ketoglutarate (KG) to succinyl-CoA by releasing reduced nicotinamide adenine dinucleotide (NADH). It is the rate-limiting reaction of the TCA cycle. This reaction contributes to the electrons of the respiratory chain and requires thiamine pyrophosphate as a cofactor. The reduction of NAD (nicotinamide adenine dinucleotide) is observed to determine its reaction rate. α-KGDH from porcine has an optimum pH range of 6.6–7.4. This enzyme is inhibited by oxidative stress and results in a metabolic deficiency. However, α-KGDH is also known to produce reactive oxygen species (ROS) leading to oxidative stress. Defective or limited levels of α-KGDH cause several neurodegenerative diseases such as Alzheimer′s disease. |
| Biochem/physiol Actions: | α-Ketoglutarate dehydrogenase is most responsive to alterations in the tumor microenvironment and contributes to the adaptive metabolic response in cancer. Inhibiting α-ketoglutarate dehydrogenase counteracts lung metastasis associated with breast cancer. |
| General description: | Research Area: Neuroscience α-Ketoglutarate dehydrogenase (α-KGDH) is a multienzyme complex localized to the mitochondria. This integrated enzyme is made up of many units of thiamine pyrophosphate-dependent dehydrogenase (E1), dihydrolipoamide dehydrogenase (E3), and dihydrolipoamide succinyl transferase (E2). |
| Legal Information: | Triton is a trademark of The Dow Chemical Company or an affiliated company of Dow |
| Other Notes: | One unit will convert 1.0 μmole of β-NAD to β-NADH per min at pH 7.4 at 30 °C in the presence of saturating levels of coenzyme A. |
| Physical form: | Supplied as a 50% glycerol solution containing ~9 mg per mL bovine serum albumin, 30% sucrose, 1.5 mM EDTA, 1.5 mM EGTA, 1.5 mM 2-mercaptoethanol, 0.3% TRITON™ X-100, 0.003% sodium azide, and 15 mM potassium phosphate, pH 6.8. |
| Hazard statements | H412 |
| Precautionary statements | P273 - P501 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| activity | specific activity: 0.1-1.0 units/mg protein (Lowry) |
| Storage Temp. | −20°C |
| Enzyme Commission (EC) Number | 1.2.4.2 ( BRENDA  | IUBMB ) |
| UNSPSC | 12352204 |