Anti-Potassium Channel Kv11.1 (HERG) Extracellular−FITC antibody produced in rabbit
SIGMA/K0640 - affinity isolated antibody, lyophilized powder
Synonym: Anti-KCNH2; Anti-Voltage gated K+ channel subfamily H member 2; Anti-
MDL Number: MFCD02097212
Product Type: Chemical
FACS analysis of live intact K562 (human chronic myelogenous leukemia) and Jurkat (human T cell leukemia) cells. Peaks at lower FL1-H values are unstained cells. Peaks at higher FL1-H values are Cells + Anti-Kv11.1-Extracellular-FITC
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| conjugate | FITC conjugate |
| form | lyophilized powder |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | flow cytometry: 2-10 μg/mL using 1x106 human live cells |
| immunocytochemistry: 1:25 using intact live HEK-KV11.1 transfected cells | |
| UniProt accession no. | Q12809  |
| Application: | Flow cytometry: a recommended working concentration of 2-10 μg per 1x106cells was determined using intact live human cells. |
| Biochem/physiol Actions: | The gene Kv11.1, also referred to as HERG (human ether-a-go-go related gene), encodes the α-subunit of the rapid component of the delayed rectifier potassium channel. The third repolarization of the action potential of human cardiomyocytes is carried out by this protein. Mutations in this gene have been associated with type II hereditary long QT syndrome (LQT2). This disease is characterized by prolongation of the QT interval, abnormal T wave, torsade de pointes, syncope and sudden death caused by cardiac arrest. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Anti-Potassium Channel KV11.1 (HERG)- Extracellular-FITC (Voltage gated K+ channel subfamily H member 2, KCNH2, ether-a-go-go-related channel 1) is produced in rabbit using as immunogen the peptide AFLLKETEEGPPATEC corresponding to residues 430-445 of human KV11.1 (HERG). Anti-KV11.1 (HERG) antibody is directed against an extracellular epitope located between the S1 and S2 domains. The antibody is affinity purified on immobilized antigen and labeled with fluorescein isothiocyanate (FITC). |
| General description: | The gene Kv11.1, also referred to as KCNH2 (potassium voltage-gated channel subfamily H member 2), is mapped to human chromosome 7. The encoded voltage-activated potassium channel is a member of the ether-a-go-go (EAG) family. |
| Immunogen: | peptide AFLLKETEEGPPATEC corresponding to residues 430-445 of human KV11.1 (HERG). |
| Other Notes: | Lyophilized powder can be stored intact at room temperature for several weeks. For extended storage, it should be stored at -20 °C or below. The reconstituted solution can be stored at 2-8 °C for up to 2 weeks. For longer storage, freeze in working aliquots. Avoid repeated freezing and thawing. Storage in “frost-free” freezers is not recommended. Centrifuge before use. Working dilution samples should be discarded if not used within 12 hours. The antibody is stable for at least 12 months when stored appropriately. |
| Physical form: | Lyophilized from phosphate buffered saline, pH 7.4, containing 1% BSA and 0.05% sodium azide. |
| Preparation Note: | Reconstitute the lyophilized vial with 50 μL or 200 μL deionized water, depending on package size. Further dilutions should be made using a carrier protein such as BSA (1-3%). |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |