Monoclonal Anti-Human IgG1 (Fab specific) [G1m(f)] antibody produced in mouse
SIGMA/I5385 - clone SG-16, ascites fluid
MDL Number: MFCD00164656
Product Type: Chemical
ELISA Monoclonal Anti-Human IgG1 (Fab specific) [G1m(f)] antibody produced in Mouse, Clone: SG-16 (Cat. No. I5385), followed by 1:1,000 Anti-Mouse IgG (Fab specific)-Peroxidase antibody produced in Goat (Cat. No. A9917), was serially diluted to detect Human IgG1 (Fab specific) by indirect ELISA. Reactivity is observed with Human IgG, Human IgG1, Human Fab and Human IgG4; but not with Human Fc, Human Light Chain, Human IgG2 and Human IgG3.
| antibody form | ascites fluid |
| antibody product type | secondary antibodies |
| biological source | mouse |
| clone | SG-16, monoclonal |
| conjugate | unconjugated |
| contains | 15 mM sodium azide |
| isotype | IgG1 |
| Quality Level | 200  |
| shipped in | dry ice |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | indirect ELISA: 1:5,000 |
| Application: | Monoclonal anti-Human IgG1 may be used for the identification of human IgG1 subclass by means of various immunoassays. It can be used in direct hemagglutination (HA) and hemagglutination inhibition (HAI) assays, immunofluorometric assay (IFMA) and detection of cytoplasmic IgG. Monoclonal anti-human IgG1 has been used in enzyme linked immunosorbent assay (ELISA). |
| Biochem/physiol Actions: | Human IgGs regulate immunological responses to allergy and pathogenic infections. IgGs have also been implicated in complement fixation and autoimmune disorders This product clone recognizes the G1m(f) allotype and has been established as a useful human IgG1 specificity standard by the WHO/IUIS study. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Monoclonal Anti-Human IgG1 (mouse IgG1isotype) is derived from the hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse. Human IgGs are glycoprotein antibodies that contain two equivalent light chains and a pair of identical heavy chains. IgGs have four distinct isoforms, ranging from IgG1 to IgG4. |
| Immunogen: | Purified human IgG |
| Hazard Codes | Xn |
| Risk Statements | 21/22 |
| Safety Statements | 36/37-60 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |