IL-3 from mouse
SIGMA/I5286 - Carrier free, recombinant, expressed in E. coli, ≥98% (SDS-PAGE), suitable for cell culture
Synonym: HCGF P-cell stimulation factorIL-3 MGC79398; Interleukin-3; MCGF (Mast cell growth factor)Multi-CSF; MGC79399
MDL Number: MFCD00164817
Product Type: Chemical
SDS-PAGE IL-3 from Mouse (Cat. No. I5286) was assessed on SDS-PAGE.
Bioassay EC50 is defined as the effective concentration of IL-3 that elicits a 50% attachment factor in cell based bioassay.
| assay | ≥98% (SDS-PAGE) |
| biological source | mouse |
| color | white |
| form | lyophilized |
| impurities | <0.1 EU/μg endotoxin, tested |
| mol wt | 15.1 kDa |
| packaging | pkg of 10 μg |
| Quality Level | 200  |
| recombinant | expressed in E. coli |
| shipped in | dry ice |
| storage temp. | −20°C |
| technique(s) | cell culture | mammalian: suitable |
| UniProt accession no. |  |
| P01586 |
| Biochem/physiol Actions: | IL-3 is a hematopoietic growth factor that promotes the survival, differentiation and proliferation of committed progenitor cells of the megakaryocyte, granulocyte-macrophage, erythroid, eosinophil, basophil and mast cell lineages. Recombinant murine IL-3 is a 15.1 kDa globular protein containing 135 amino acid residues. |
| General description: | IL-3 is a hematopoietic growth factor that promotes the survival, differentiation and proliferation of committed progenitor cells of the megakaryocyte, granulocyte-macrophage, erythroid, eosinophil, basophil and mast cell lineages. Produced by T cells, mast cells and eosinophils, IL-3 enhances thrombopoieses, phagocytosis, and antibody-mediated cellular cytotoxicity. Its ability to activate monocytes suggests that IL-3 may have additional immunoregulatory roles. Many of the IL-3 activities depend upon co-stimulation with other cytokines. IL-3 is species-specific, variably glycosylated cytokine. Recombinant murine IL-3 is a 15.1 kDa globular protein containing 135 amino acid residues. |
| Preparation Note: | Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a stabilizer (example 5% Trehalose) and store in working aliquots at -20°C to -80°C. |
| Hazard Codes | Xi |
| Risk Statements | 36/37/38 |
| Safety Statements | 26 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 2 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | ≥98% (SDS-PAGE) |
| Storage Temp. | −20°C |
| UNSPSC | 12352202 |