Anti-INCENP antibody produced in rabbit
SIGMA/I5283 - IgG fraction of antiserum, buffered aqueous solution
Synonym: Anti-Inner Centromere Protein
MDL Number: MFCD05665235
Product Type: Chemical
Immunofluorescence HeLa cells were fixed and permeabilized with 4% paraformaldehyde followed by cold methanol. Fixed cells were stained with 1:100 Anti-INCENP antibody produced in Rabbit (Cat. No. I5283) followed by 1:100 Anti-Rabbit IgG (whole molecule)-FITC antibody produced in Goat (Cat. No. F9887).
Western Blotting Whole extract of NIH-3T3 Mouse fibroblasts cells were separated on SDS-PAGE and probed with Anti-INCENP antibody produced in Rabbit (Cat. No. I5283). The antibody was developed using 1:10,000 Anti-Rabbit IgG (whole molecule)-Peroxidase antibody produced in Goat (Cat. No. A0545). Lanes 1. 1:2,000 antibody 2. 1:4,000 antibody 3. Negative Control
| antibody form | IgG fraction of antiserum |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| mol wt | antigen ~106 kDa |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | human, mouse, rat |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | indirect immunofluorescence: 1:100 using HeLa cells |
| microarray: suitable | |
| western blot (chemiluminescent): 1:2,000 using whole extract of mouse NIH3T3 and rat PC12 cells | |
| western blot (chemiluminescent): 1:5,000 using nuclear extract of human HeLa cells | |
| UniProt accession no. | Q9NQS7  |
| Application: | Anti-INCENP antibody produced in rabbit has been used in western blotting and immunofluorescence. |
| Biochem/physiol Actions: | Studies in knockout mice established INCENP (inner centromere protein) as essential for mouse development and viability. Loss of INCENP results in chromosome segregation defects and failures in cytokinesis. |
| Biochem/physiol Actions: | The inner centromere protein encoded by the INCENP gene is phosphorylated by Cdk1 (cyclin dependent kinase 1) in mitosis. It is found to be associated with Plk1 (Polo-like kinase) in a polo-box–dependent manner. Plk1 is a serine/threonine kinase that functions in cell cycle, especially in mitosis. INCEP participates in the localization of Bub1 (Budding uninhibited by benzimidazole 1), a serine/threonine kinase involved in spindle checkpoint, to kinetochore. This, in turn, promotes the recruitment of Plk1 to kinetochores. Phosphorylated INCENP stimulates the activity of Aurora B kinase at centromeres and the central spindle that is important for chromosome segregation and cytokinesis, respectively. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | The gene INCENP (inner centromere protein) encodes an inner centromere protein that forms a component of the chromosomal passenger complex (CPC). It is found to be localized to inner centromeres during mitosis. The CPC also contains Aurora B, survivin, and Borealin. |
| Immunogen: | synthetic peptide corresponding to amino acid residues 884-901 of human INCENP with N-terminal added cysteine conjugated to KLH. The corresponding sequence differs by one amino acid in chicken and Xenopus laevis and by two amino acids in mouse. |
| Packaging: | 200 μL in glass bottle |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 2 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |