Interleukin-1β from mouse
SIGMA/I5271 - IL-1β, recombinant, expressed in E. coli, lyophilized powder, suitable for cell culture
Synonym: IL-1β; Interleukin-1β; Lymphocyte activating factor; IL-1β; Lymphocyte activating factor
MDL Number: MFCD00164815
Product Type: Chemical
Bioassay for Interleukin 1 β The biological activity of recombinant mouse IL-1β (Cat. No. I5271) is measured by the dose-dependent stimulation of murine D10G4.1 cells. The ED50 is defined as the effective concentration of growth factor that elicits a 50% increase in cell growth in a cell based bioassay.
| assay | ≥97% (SDS-PAGE) |
| biological source | mouse |
| form | lyophilized powder |
| impurities | ≤0.1ng/mg |
| mol wt | 17.5 kDa (containing 153 amino acid residues) |
| packaging | pkg of 5 μg |
| pkg of 5X5 μg | |
| potency | ≤0.010 ng/mL ED50 |
| quality | endotoxin tested |
| Quality Level | 200  |
| recombinant | expressed in E. coli |
| storage temp. | −20°C |
| technique(s) | cell culture | mammalian: suitable |
| UniProt accession no. | P10749  |
| Analysis Note: | The biological activity is measured by the dose-dependent stimulation of murine D10G4.1 cells. |
| Application: | Interleukin-1β from mouse has been used: • as a proinflammatory agent, to induce inflammatory signaling cascades mediated cellular damage • to study its effect on fetuin A-induced inflammatory response in podocyte • to check its effect on the expression of mitogen-activated protein kinase 4 using 3T3-L1 adipocytes |
| Biochem/physiol Actions: | Interleukin-1β (IL-1β) is a potent inflammatory cytokine, and mediates inflammation through proinflammatory molecules: cyclooxygenase-2 (COX2) and nitric oxide. IL-1β release is stimulated by injury and increased levels are observed in response to severe pain. It has its main function in T-cell activation, antigen recognition and T-helper cell 17 cell development. IL-1β is also responsible for the formation of IL-17 and IL-22 by nature killer T (NKT) cells and NK cells. It is known to induce inflammation in psoriasis and rheumatoid arthritis. IL-1β controls intercellular adhesion molecule-1 expression. It is significantly associated with bone damage and vascular injury. |
| General description: | Interleukin-1β (IL-1β) cytokine is generated by monocytes, dendritic cells, macrophages and keratinocytes. Pro-IL-1β, an IL-1β precursor undergoes proteolytic cleavage to produce its active form of 17 kDa. |
| Other Notes: | The two closely related agents, Interleukin-1α (I |
| Physical form: | Lyophilized from 0.2 μm filtered solution in 10mM Sodium Citrate, pH 4.0 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | ≥97% (SDS-PAGE) |
| Storage Temp. | −20°C |
| UNSPSC | 12352202 |