Anti-Human IgG (Fab specific) antibody produced in goat
SIGMA/I5260 - affinity isolated antibody, buffered aqueous solution
Synonym: Fab-Specific Antibody
MDL Number: MFCD00162431
Product Type: Chemical
ELISA Anti-Human IgG (Fab specific) antibody produced in Goat (Cat. No. I5260), followed by 1:5,000 Anti-Goat IgG (whole molecule)-Peroxidase antibody produced in Rabbit (Cat. No. A5420), was serially diluted to detect Human IgG by indirect ELISA.
| antibody form | affinity isolated antibody |
| antibody product type | secondary antibodies |
| biological source | goat |
| clone | polyclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| Quality Level | 300  |
| shipped in | dry ice |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | indirect ELISA: 1:50,000 |
| quantitative precipitin assay: 2.0 mg/mL |
| Application: | Anti-Human IgG (Fab specific) antibody produced in goat is effective as a second antibody reagent in immunoassay procedures. It may be used: • as starting material for conjugates using enzymes or fluorescent dyes • in enzyme linked immunosorbent assay • as a cross-linking antibody in internalisation of 125I-IgG by streptolysin-O-permeabili • as capture antibody in enzyme-linked immunosorbent assay (ELISA) for detection and semiquantitation of Fab • in electrophoresis and immunoelectrophoresis |
| Biochem/physiol Actions: | Enzymatic cleavage of IgG yields antigen-binding (Fab) and effector activating (Fc) fragments. IgG treatment with papain produces two Fab fragments and one Fc fragment. IgG treatment with pepsin produces (Fab′)2 fragment alone. IgG antibodies regulate several functions such as complement activation and phagocytosis. Thus they play a crucial role in facilitating cytological immune responses. Anti-human IgG (Fab specific) antibody can be used in Ouchterlony double diffusion. Due to lack of interspecies cross reactivity to mouse or rat ascites fluids, this antibody is also ideal for screening human monoclonal antibodies produced by hybridoma cells grown in vivo. |
| Biochem/physiol Actions: | Goat anti-human IgG (Fab specific) antibody reacts specifically with Fab fragment of human IgG and shows no reactivity for Fc fragment of human IgG. This product has no interspecies cross reactivity for mouse and rat IgG. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Anti-Human IgG (Fab specific) antiserum is produced in goat using purified human IgG Fab fragment as the immunogen. Affinity isolated antibody is obtained from goat anti-human IgG antiserum by immunospecific purification which removes essentially all goat serum proteins, including immunoglobulins, which do not specifically bind to the Fab fragment of human IgG. IgG is a larger molecule with a molecular weight of 150 kilo daltons (kDa). IgG has two light chains (L) and two heavy chains (H). The light chain can be either lambda (λ) or kappa (κ) chain. Functionally, immunoglobulins have constant region and variable region. Light chain has one variable region in the N-terminal VL and followed by one constant domain CL. The light chain is approximately about 25 kDa. The heavy chain has one variable region in the N-terminal and followed by three constant domains. In between the first (C1H) and second constant (C2H) domain in the spacer hinge region which connects both the heavy chains. Each heavy chain is about 55 kDa. |
| Immunogen: | Purified human IgG Fab fragment. |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide as preservative |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |