Ionomycin calcium salt from Streptomyces conglobatus

SIGMA/I0634 - powder, ≥98% (HPLC)

Synonym: Ionomycin (calcium); SQ23377 calcium

CAS Number: 56092-82-1
Empirical Formula (Hill Notation): C₄₁H₇₀CaO₉
Molecular Weight: 747.07
MDL Number: MFCD00083634
Linear Formula: C₄₁H₇₀CaO₉
Product Type: Chemical

Catalog Number	PKG Qty.	Price


45-I0634-1MG	1 mg	$168.00 1/EA	Add To Favorites
45-I0634-5MG	5 mg	$731.00 1/EA	Add To Favorites

Calcium Ionomycin is a polyether antibiotic produced by Streptomyces conglobatus (ATCC 31005). Calcium Ionomycin is capable of extracting Ca2+ and other divalent cations from an aqueous into an organic phase. Calcium salt of ionomycin is an effective mobile Ca + carrier and it has significant advantages for use in studies of Ca2+ transport across biological membranes. Calcium Ionomycin can serve as an inducer of apoptosis, which was suggested to act by activation of a latent, calcium-responsive endonuclease.

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Properties
Descriptions
Safety Info.
Basic Data

antibiotic activity spectrum	Gram-positive bacteria
assay	≥98% (HPLC)
color	white to off-white
form	powder
InChI	1S/C41H72O9.Ca/c1-25(21-29(5)34(43)24-35(44)30(6)22-27(3)20-26(2)14-15-38(46)47)12-11-13-28(4)39(48)31(7)36(45)23-33-16-18-41(10,49-33)37-17-19-40(9,50-37)32(8)42;/h11,13,24-33,36-37,39,42-43,45,48H,12,14-23H2,1-10H3,(H,46,47);/q;+2/p-2/b13-11+,34-24-;/t25-,26-,27+,28-,29-,30+,31+,32-,33+,36+,37-,39-,40+,41+;/m1./s1
InChI key	WKRWUYKLUMMAKG-WYGBAUISSA-L
mode of action	cell membrane \| interferes
Quality Level	300
SMILES string	C[C@@H]1CCC(=O)O[Ca]OC(=CC(=O)[C@@H](C)C[C@@H](C)C1)[C@H](C)C[C@H](C)CC=C[C@@H](C)[C@@H](O)[C@@H](C)[C@@H](O)C[C@@H]2CC[C@](C)(O2)[C@H]3CC[C@](C)(O3)[C@@H](C)O
solubility	DMSO: 10 mg/mL
	ethanol: soluble
storage condition	desiccated
	protect from light
storage temp.	2-8°C

Application:	Ionomycin calcium salt from Streptomyces conglobatus has been used as a media supplement to culture: peripheral blood mononuclear cells (PBMCs) for immunologic assessment oocytes for parthenogenetic activation T cell subsets for the detection of intracellular cytokines, carboxyfluorescein diacetate succinimidyl ester (CFSE) proliferation assay, and antigen-specific suppressive function assay in combination with phorbol12-myristate 13-acetate (PMA) to stimulate T-cell activation in various cells as sperm capacitation medium supplement to study its impact on in vitro fertilization and increasing the efficiency of bovine embryo development as a positive control for the detection of intracellular Ca²⁺ in adipose-derived stem cells (ASCs)(4)
Biochem/physiol Actions:	Ca²⁺ ionophore that is more effective than A23187 as a mobile ion carrier for Ca²⁺.
Biochem/physiol Actions:	Ca²⁺ ionophore that is more effective than A23187 as a mobile ion carrier for Ca²⁺; non-fluorescent; used to study Ca²⁺ transport across biological membranes; induces apoptotic degeneration of embryonic cortical neurons.
Biochem/physiol Actions:	Ionomycin is an ionophore, widely used for validation and calibration of calcium analysis.
General description:	Calcium Ionomycin is a polyether antibiotic made by Streptomyces conglobatus. It can remove Ca²⁺ and divalent cations from an aqueous into an organic phase. Ionomycin enhances the influx of Ca²⁺ by triggering store-regulated cation entry rather than acting directly on the plasma membrane. It can induce apoptosis, potentially by activating a hidden enzyme that responds to calcium. Since calcium salt of ionomycin serves as a highly efficient transporter of Ca²⁺, it offers substantial benefits for studying the transport of Ca²⁺ across biological membranes. Ionomycin is employed to balance intracellular and extracellular calcium ion concentrations for in situ calibrations of fluorescent indicators. Additionally, it is a narrow spectrum antibiotic effective against Gram-positive bacteria, obtained in pure form from the fermentation broths of Streptomyces conglobatus. It is unique amongst known polyether antibiotics due to its exceptional UV absorption maximum at 300 nm and its high affinity for calcium ions.
Other Notes:	2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research
Packaging:	1, 5 mg in glass bottle
Preparation Note:	Store ionomycin in powder form desiccated and protected from light at 2-8 °C. Under these conditions the product is stable for 3 years. Store stock solutions in ethanol or DMSO at -20 °C, protected from light. Under these conditions the stock solutions are stable for several months. For short term use (up to 6 weeks) the stock solutions maybe stored at 2-8 °C protected from light.

Symbol	GHS07
Signal word	Warning
Hazard statements	H302
Precautionary statements	P264 - P270 - P301 + P312 - P501
Hazard Codes	Xn
Risk Statements	22
Safety Statements	36
RIDADR	NONH for all modes of transport
WGK Germany	WGK 3
Flash Point(F)	Not applicable
Flash Point(C)	Not applicable

Purity	≥98% (HPLC)
Storage Temp.	2-8°C
UNSPSC	12352200

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