Formalin solution, neutral buffered, 10%
SIGMA/HT5012 - case of 24 × 60 mL, histological tissue fixative
Synonym: 10% NBF; 10% Neutral Buffered Formalin; Formaldehyde; Methanal
MDL Number: MFCD00003274
Product Type: Chemical
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| application(s) | hematology histology |
| biological source | synthetic |
| color | colorless |
| concentration | 10% formalin (approx. 4% formaldehyde) |
| density | 1.080 g/cm3 |
| InChI | 1S/CH2O/c1-2/h1H2 |
| InChI key | WSFSSNUMVMOOMR-UHFFFAOYSA |
| packaging | case of 24 × 60 mL |
| pH | 6.90-7.10 |
| Quality Level | 200  |
| SMILES string | [O-]#[C+H2] |
| storage temp. | room temp |
| Application: | Formalin solution (neutral buffered, 10%) has been popularly used as a general-purpose histological fixative to fix tissue samples.• It effectively preserves the cellular and tissue morphology, maintaining the structural integrity required for microscopic examination. • It is used for routine histological staining, providing clear and detailed images of cellular structures. |
| Application: | Popular general purpose histological fixative. |
| Biochem/physiol Actions: | Formaldehyde is both a non-coagulant and an additive fixative. Formalin fixation is thought to form cross links between the aldehydes and the proteins, creating a gel, thus retaining cellular constituents in their in vivo relationship. Formaldehyde is known to penetrate tissue quickly, but the fixation process may take a long time to form the various cross links with tissue proteins. |
| Features and Benefits: | • 10% NBF is well-suited for large throughput laboratories. • It requires a relatively short period of fixation. • It can be used for the long-term storage of tissue. |
| General description: | 10% Neutral Buffered Formalin is the most used fixative throughout the world for light microscopy. Formalin solution is the commercially obtained solution containing 37% w/w (40% w/v) of formaldehyde in water. It requires a relatively short period of fixation. It is suitable for the long-term storage of tissue. Once properly fixed, the tissue should be able to withstand the subsequent stages of tissue processing or staining. |
| Packaging: | Case containers are polypropylene with white leak-proof lids and are partially prefilled with 10% NBF. |
| Physical form: | Neutral buffered solution |
| Symbol |   GHS07,GHS08 |
| Signal word | Danger |
| Hazard statements | H302 - H317 - H341 - H350 |
| Precautionary statements | P201 - P280 - P301 + P312 + P330 - P302 + P352 - P308 + P313 |
| Hazard Codes | T |
| Risk Statements | 45-20/21/22-43-68 |
| Safety Statements | 53-36/37-45 |
| WGK Germany | WGK 3 |
| Flash Point(F) | 185.0 °F |
| Flash Point(C) | 85 °C |
| Density | 1.080 g/cm3 |
| Storage Temp. | room temp |
| UNSPSC | 12352114 |