Anti-SLC9A3 antibody produced in rabbit
SIGMA/HPA036493 - Prestige Antibodies® Powered by Atlas Antibodies, affinity isolated antibody, buffered aqueous glycerol solution
Synonym: Anti-NHE3; Anti-Solute carrier family 9 (sodium/hydrogen exchanger), member 3
Product Type: Chemical
Enhanced Validation-Orthogonal RNAseq Confirmation Immunohistochemistry analysis in human colon and liver tissues using HPA036493 antibody. Corresponding SLC9A3 RNA-seq data are presented for the same tissues.
Enhanced Validation-By Independent Antibodies Immunohistochemical staining of human colon, kidney, liver and small intestine using Anti-SLC9A3 antibody HPA036493 (A) shows similar protein distribution across tissues to independent antibody HPA036669 (B).
Immunohistochemistry Immunohistochemical staining of human gallbladder shows strong positivity in apical membrane in glandular cells.
Immunohistochemistry Immunohistochemical staining of human colon shows strong positivity in apical membrane in glandular cells.
Immunohistochemistry Immunohistochemical staining of human small intestine shows moderate positivity in apical membrane in glandular cells.
Immunohistochemistry Immunohistochemical staining of human kidney shows moderate positivity in apical membrane in cells in tubules.
Immunohistochemistry Immunohistochemical staining of human liver shows no membranous positivity in hepatocytes as expected.
Immunofluorescence Immunofluorescent staining of human cell line U-2 OS shows localization to plasma membrane.
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| conjugate | unconjugated |
| enhanced validation | orthogonal RNAseq independent Learn more about Antibody Enhanced Validation  |
| form | buffered aqueous glycerol solution |
| immunogen sequence | TDNVVNVDFTPRSSTVEASVSYLLR |
| product line | Prestige Antibodies® Powered by Atlas Antibodies |
| Quality Level | 100 |
| shipped in | wet ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunofluorescence: 0.25-2 μg/mL |
| immunohistochemistry: 1:20-1:50 | |
| UniProt accession no. | P48764  |
| Application: | All Prestige Antibodies Powered by Atlas Antibodies are developed and validated by the Human Protein Atlas (HPA) project and as a result, are supported by the most extensive characterization in the industry. The Human Protein Atlas project can be subdivided into three efforts: Human Tissue Atlas, Cancer Atlas, and Human Cell Atlas. The antibodies that have been generated in support of the Tissue and Cancer Atlas projects have been tested by immunohistochemistry against hundreds of normal and disease tissues and through the recent efforts of the Human Cell Atlas project, many have been characterized by immunofluorescence to map the human proteome not only at the tissue level but now at the subcellular level. These images and the collection of this vast data set can be viewed on the Human Protein Atlas (HPA) site by clicking on the Image Gallery link. We also provide Prestige Antibodies® protocols and other useful information. |
| Biochem/physiol Actions: | Solute carrier family 9 member A3 (SLC11A1) helps in electroneutral sodium absorption in the small intestine. It maintains water and sodium homeostasis. Inhibition of SLC11A1 is linked to diarrheal diseases and sudden infant death syndrome. The protein plays a pivotal role in the pathogenesis of cholesterol gallstone disease (CGD). |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| Features and Benefits: | Prestige Antibodies® are highly characterized and extensively validated antibodies with the added benefit of all available characterization data for each target being accessible via the Human Protein Atlas portal linked just below the product name at the top of this page. The uniqueness and low cross-reactivity of the Prestige Antibodies® to other proteins are due to a thorough selection of antigen regions, affinity purification, and stringent selection. Prestige antigen controls are available for every corresponding Prestige Antibody and can be found in the linkage section. Every Prestige Antibody is tested in the following ways: • IHC tissue array of 44 normal human tissues and 20 of the most common cancer type tissues. • Protein array of 364 human recombinant protein fragments. |
| General description: | Solute carrier family 9 member A3 (SLC11A1) is a Na+/H+ exchanger. The protein is expressed on the epithelial brush border in the small intestine, colon, gall bladder, renal proximal tubule, thick ascending limb of the loop of Henle in the kidney and respiratory center. The gene is located on human chromosome 5p15.3. |
| Immunogen: | solute carrier family 9 (sodium/hydrogen exchanger), member 3 recombinant protein epitope signature tag (PrEST) |
| Legal Information: | Prestige Antibodies is a registered trademark of Merck KGaA, Darmstadt, Germany |
| Other Notes: | Corresponding Antigen APREST79938 |
| Physical form: | Solution in phosphate buffered saline, pH 7.2, containing 40% glycerol and 0.02% sodium azide. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |