Anti-PSMB1 antibody produced in rabbit
SIGMA/HPA029635 - affinity isolated antibody, buffered aqueous glycerol solution, ab1
Synonym: Anti-HC5; Anti-PMSB1; Anti-proteasome (prosome, macropain) subunit, β type, 1
Product Type: Chemical
Enhanced Validation-By Independent Antibodies Immunohistochemical staining of human colon, kidney, liver and testis using Anti-PSMB1 antibody HPA029635 (A) shows similar protein distribution across tissues to independent antibody HPA029637 (B).
Enhanced Validation-By Independent Antibodies Western blot analysis using Anti-PSMB1 antibody HPA029635 (A) shows similar pattern to independent antibody HPA029637 (B).
Immunohistochemistry Immunohistochemical staining of human colon shows strong cytoplasmic positivity in glandular cells.
Immunohistochemistry Immunohistochemical staining of human kidney shows moderate to strong cytoplasmic and nuclear positivity in cells in tubules.
Immunohistochemistry Immunohistochemical staining of human liver shows moderate to strong cytoplasmic positivity in hepatocytes.
Immunohistochemistry Immunohistochemical staining of human testis shows moderate to strong cytoplasmic and nuclear positivity in cells in seminiferous ducts.
Immunofluorescence Immunofluorescent staining of human cell line Hep G2 shows localization to nucleus.
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| conjugate | unconjugated |
| enhanced validation | independent Learn more about Antibody Enhanced Validation  |
| form | buffered aqueous glycerol solution |
| immunogen sequence | YQRDSFKAGGSASAMLQPLLDNQVG |
| Quality Level | 100 |
| shipped in | wet ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunoblotting: 0.04-0.4 μg/mL |
| immunofluorescence: 0.25-2 μg/mL | |
| immunohistochemistry: 1:500-1:1000 | |
| UniProt accession no. | P20618  |
| Application: | All Prestige Antibodies Powered by Atlas Antibodies are developed and validated by the Human Protein Atlas (HPA) project and as a result, are supported by the most extensive characterization in the industry. The Human Protein Atlas project can be subdivided into three efforts: Human Tissue Atlas, Cancer Atlas, and Human Cell Atlas. The antibodies that have been generated in support of the Tissue and Cancer Atlas projects have been tested by immunohistochemistry against hundreds of normal and disease tissues and through the recent efforts of the Human Cell Atlas project, many have been characterized by immunofluorescence to map the human proteome not only at the tissue level but now at the subcellular level. These images and the collection of this vast data set can be viewed on the Human Protein Atlas (HPA) site by clicking on the Image Gallery link. We also provide Prestige Antibodies® protocols and other useful information. |
| Biochem/physiol Actions: | Proteasome subunit beta type-1 (PSMB1) phosphorylation is critical for its localization in nucleus. Theophylline (THP) and 1,3-dinitrobenzene (DNB) regulates expression of PSMB1 and other proteins in the ubiquitin-proteasome pathway. Polymorphism in PSMB1 impacts the protease activity of proteasomes and improves effect of the anti-cancer drug, bortezomib. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| Features and Benefits: | Prestige Antibodies® are highly characterized and extensively validated antibodies with the added benefit of all available characterization data for each target being accessible via the Human Protein Atlas portal linked just below the product name at the top of this page. The uniqueness and low cross-reactivity of the Prestige Antibodies® to other proteins are due to a thorough selection of antigen regions, affinity purification, and stringent selection. Prestige antigen controls are available for every corresponding Prestige Antibody and can be found in the linkage section. Every Prestige Antibody is tested in the following ways: • IHC tissue array of 44 normal human tissues and 20 of the most common cancer type tissues. • Protein array of 364 human recombinant protein fragments. |
| General description: | Proteasome subunit beta type-1 (PSMB1) is part of the 20S proteasome subunit complex. PSMB1 is mapped to human chromosome 6q27. |
| Immunogen: | proteasome (prosome, macropain) subunit, beta type, 1 recombinant protein epitope signature tag (PrEST) |
| Legal Information: | Prestige Antibodies is a registered trademark of Merck KGaA, Darmstadt, Germany |
| Other Notes: | Corresponding Antigen APREST77253 |
| Physical form: | Solution in phosphate-buffered saline, pH 7.2, containing 40% glycerol and 0.02% sodium azide. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |