Anti-PADI4 antibody produced in rabbit
SIGMA/HPA017007 - Prestige Antibodies® Powered by Atlas Antibodies, affinity isolated antibody, buffered aqueous glycerol solution
Synonym: Anti-HL-60 PAD; Anti-
MDL Number: MFCD09039201
Product Type: Chemical
Enhanced Validation-By Independent Antibodies Immunohistochemical staining of human bone marrow, liver, spleen and testis using Anti-PADI4 antibody HPA017007 (A) shows similar protein distribution across tissues to independent antibody HPA042825 (B).
Enhanced Validation-Orthogonal RNAseq Confirmation Immunohistochemistry analysis in human bone marrow and kidney tissues using HPA017007 antibody. Corresponding PADI4 RNA-seq data are presented for the same tissues.
By Recombinant Expression Western blot analysis in control (vector only transfected HEK293T lysate) and pADI4 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY402202).
Immunohistochemistry Immunohistochemical staining of human kidney shows no nuclear positivity as expected.
Immunohistochemistry Immunohistochemical staining of human liver shows no nuclear positivity in hepatocytes as expected.
Immunohistochemistry Immunohistochemical staining of human spleen shows strong nuclear positivity in cells in red pulp.
Immunohistochemistry Immunohistochemical staining of human testis shows no nuclear positivity in cells in seminiferous ducts as expected.
Immunohistochemistry Immunohistochemical staining of human bone marrow shows strong nuclear positivity in hematopoietic cells.
Immunohistochemistry Immunohistochemical staining of human bone marrow shows strong nuclear positivity in hematopoietic cells.
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| conjugate | unconjugated |
| enhanced validation | recombinant expression independent orthogonal RNAseq Learn more about Antibody Enhanced Validation  |
| form | buffered aqueous glycerol solution |
| immunogen sequence | CEDDEVLDSEDLQDMSLMTLSTKTP |
| product line | Prestige Antibodies® Powered by Atlas Antibodies |
| Quality Level | 100 |
| shipped in | wet ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunoblotting: 0.04-0.4 μg/mL |
| immunohistochemistry: 1:50-1:200 | |
| UniProt accession no. | Q9UM07  |
| Application: | Anti-PADI4 antibody produced in rabbit, a Prestige Antibody, is developed and validated by the Human Protein Atlas (HPA) project . Each antibody is tested by immunohistochemistry against hundreds of normal and disease tissues. These images can be viewed on the Human Protein Atlas (HPA) site by clicking on the Image Gallery link. The antibodies are also tested using immunofluorescence and western blotting. To view these protocols and other useful information about Prestige Antibodies and the HPA, visit sigma.com/prestige . |
| Biochem/physiol Actions: | PADI4 (peptidyl arginine deiminase, type IV) is mainly involved in the Arg methylation and citrullination in histones. It binds at the histones H3 and H4 positions to convert methylated-Arg to citrulline by releasing methylamine in presence of Ca2+. It also acts as the transcriptional regulator of estrogen-responsive genes. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| Features and Benefits: | Prestige Antibodies® are highly characterized and extensively validated antibodies with the added benefit of all available characterization data for each target being accessible via the Human Protein Atlas portal linked just below the product name at the top of this page. The uniqueness and low cross-reactivity of the Prestige Antibodies® to other proteins are due to a thorough selection of antigen regions, affinity purification, and stringent selection. Prestige antigen controls are available for every corresponding Prestige Antibody and can be found in the linkage section. Every Prestige Antibody is tested in the following ways: • IHC tissue array of 44 normal human tissues and 20 of the most common cancer type tissues. • Protein array of 364 human recombinant protein fragments. |
| General description: | PADI4 (peptidyl arginine deiminase, type IV) is a member of the citrullinating enzymes involved in the conversion of protein-bound arginine to citrulline. |
| Immunogen: | Protein-arginine deiminase type-4 recombinant protein epitope signature tag (PrEST) |
| Legal Information: | Prestige Antibodies is a registered trademark of Merck KGaA, Darmstadt, Germany |
| Other Notes: | Corresponding Antigen APREST70022 |
| Physical form: | Solution in phosphate-buffered saline, pH 7.2, containing 40% glycerol and 0.02% sodium azide |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |