Anti-ASGR2 antibody produced in rabbit
SIGMA/HPA014899 - Prestige Antibodies® Powered by Atlas Antibodies, affinity isolated antibody, buffered aqueous glycerol solution
Synonym: Anti-ASGP-R 2; Anti-ASGPR 2; Anti-
Product Type: Chemical
Enhanced Validation-Orthogonal RNAseq Confirmation Immunohistochemistry analysis in human liver and pancreas tissues using Anti-ASGR2 antibody. Corresponding ASGR2 RNA-seq data are presented for the same tissues.
Enhanced Validation-By Independent Antibodies Immunohistochemical staining of human colon, liver, lymph node and pancreas using Anti-ASGR2 antibody HPA014899 (A) shows similar protein distribution across tissues to independent antibody HPA015998 (B).
Immunohistochemistry Immunohistochemical staining of human liver shows high expression.
Immunohistochemistry Immunohistochemical staining of human pancreas shows low expression as expected.
Immunohistochemistry Immunohistochemical staining of human colon using Anti-ASGR2 antibody HPA014899.
Immunohistochemistry Immunohistochemical staining of human liver using Anti-ASGR2 antibody HPA014899.
Immunohistochemistry Immunohistochemical staining of human lymph node using Anti-ASGR2 antibody HPA014899.
Immunohistochemistry Immunohistochemical staining of human pancreas using Anti-ASGR2 antibody HPA014899.
Western Blotting Western blot analysis in human cell line HepG2.
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| conjugate | unconjugated |
| enhanced validation | independent orthogonal RNAseq recombinant expression Learn more about Antibody Enhanced Validation  |
| form | buffered aqueous glycerol solution |
| immunogen sequence | QSAQLQAELRSLKEAFSNFSSSTLT |
| product line | Prestige Antibodies® Powered by Atlas Antibodies |
| Quality Level | 100 |
| shipped in | wet ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunoblotting: 0.04-0.4 μg/mL |
| immunohistochemistry: 1:5000-1:10000 | |
| UniProt accession no. | P07307  |
| Application: | All Prestige Antibodies Powered by Atlas Antibodies are developed and validated by the Human Protein Atlas (HPA) project and as a result, are supported by the most extensive characterization in the industry. The Human Protein Atlas project can be subdivided into three efforts: Human Tissue Atlas, Cancer Atlas, and Human Cell Atlas. The antibodies that have been generated in support of the Tissue and Cancer Atlas projects have been tested by immunohistochemistry against hundreds of normal and disease tissues and through the recent efforts of the Human Cell Atlas project, many have been characterized by immunofluorescence to map the human proteome not only at the tissue level but now at the subcellular level. These images and the collection of this vast data set can be viewed on the Human Protein Atlas (HPA) site by clicking on the Image Gallery link. We also provide Prestige Antibodies® protocols and other useful information. |
| Biochem/physiol Actions: | ASGR2 (asialoglycoprotein receptor 2) forms a subunit of ASGR, which internalizes desialylated glycoproteins, and transports them to lysosomes. It is also a receptor for hepatitis B virus (HBV), the chronic infection of which can affect the male reproductive system. As ASGR is also expressed in human testis, it might be responsible for HBV infection in the luminal compartment of testis. This receptor is also involved in the immune regulation and inflammatory processes in hepatocytes, and promotes liver cell toxicity. An alternatively spliced form of ASGR2 subunit, called sH2a is found in serum of healthy individuals. The serum level of this form is changed in liver fibrosis, and defines the status of hepatocyte function. Thus, it has potential as a non-invasive marker for liver function and fibrosis. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| Features and Benefits: | Prestige Antibodies® are highly characterized and extensively validated antibodies with the added benefit of all available characterization data for each target being accessible via the Human Protein Atlas portal linked just below the product name at the top of this page. The uniqueness and low cross-reactivity of the Prestige Antibodies® to other proteins are due to a thorough selection of antigen regions, affinity purification, and stringent selection. Prestige antigen controls are available for every corresponding Prestige Antibody and can be found in the linkage section. Every Prestige Antibody is tested in the following ways: • IHC tissue array of 44 normal human tissues and 20 of the most common cancer type tissues. • Protein array of 364 human recombinant protein fragments. |
| General description: | ASGR2 (asialoglycoprotein receptor 2) is one of the two subunits of the hetero-oligomeric ASGP-R, which is expressed specifically on hepatocytes. It is also called H2 and shares high homology with the other subunit called H1. ASGP-R is present on the plasma membrane of hepatocytes, towards the sinusoidal side, and undergoes endocytic recycling. This receptor is a type II membrane protein, and thus, ASGR2 has its N-terminal towards the cytoplasm, one transmembrane region, a stalk region and a Ca2+-dependent CRD (carbohydrate recognition domain). The molecular weight of this subunit is ~ 50kDa. |
| Immunogen: | Asialoglycoprotein receptor 2 recombinant protein epitope signature tag (PrEST) |
| Legal Information: | Prestige Antibodies is a registered trademark of Merck KGaA, Darmstadt, Germany |
| Other Notes: | Corresponding Antigen APREST72295 |
| Physical form: | Solution in phosphate-buffered saline, pH 7.2, containing 40% glycerol and 0.02% sodium azide |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |