Anti-ILF3 antibody produced in rabbit
SIGMA/HPA001897 - Prestige Antibodies® Powered by Atlas Antibodies, affinity isolated antibody, buffered aqueous glycerol solution
Synonym: Anti-DRBP76 antibody produced in rabbit; Anti-Double-stranded RNA-binding protein 76 antibody produced in rabbit; Anti-Interleukin enhancer-binding factor 3 antibody produced in rabbit; Anti-NF-AT-90 antibody produced in rabbit; Anti-NFAR antibody produced in rabbit; Anti-Nuclear factor associated with dsRNA antibody produced in rabbit; Anti-Nuclear factor of activated T-cells 90 kDa antibody produced in rabbit; Anti-TCP80 antibody produced in rabbit; Anti-Translational control protein 80 antibody produced in rabbit
Product Type: Chemical
Immunohistochemistry Immunohistochemical staining of human lymph node shows strong nuclear positivity in cells in seminiferous ducts.
Immunohistochemistry Immunohistochemical staining of human lymph node shows strong nuclear positivity in germinal center cells.
Immunohistochemistry Immunohistochemical staining of human cerebral cortex shows strong nuclear positivity in neurons.
Immunohistochemistry Immunohistochemical staining of human pancreas shows strong nuclear positivity in exocrine glandular cells.
Immunofluorescence Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm, nucleoli and mitochondria.
Western Blotting Western blot analysis in human cell line MOLT-4.
Prestige Antibodies® Highly characterized and Enhanced Validated antibodies
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| conjugate | unconjugated |
| form | buffered aqueous glycerol solution |
| immunogen sequence | VMAKHSSVYPTQEELEAVQNMVSHT |
| product line | Prestige Antibodies® Powered by Atlas Antibodies |
| Quality Level | 100  |
| shipped in | wet ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunoblotting: 0.04-0.4 μg/mL |
| immunofluorescence: 0.25-2 μg/mL | |
| immunohistochemistry: 1:2500-1:5000 | |
| UniProt accession no. | Q12906  |
| Application: | Anti-ILF3 antibody produced in rabbit, a Prestige Antibody, is developed and validated by the Human Protein Atlas (HPA) project . Each antibody is tested by immunohistochemistry against hundreds of normal and disease tissues. These images can be viewed on the Human Protein Atlas (HPA) site by clicking on the Image Gallery link. The antibodies are also tested using immunofluorescence and western blotting. To view these protocols and other useful information about Prestige Antibodies and the HPA, visit sigma.com/prestige . |
| Biochem/physiol Actions: | Exportin-5 mediates the transport of ILF3 (interleukin enhancer binding factor 3) from nucleus to cytoplasm. VA1 RNA, exportin-5, ILF3 and RanGTP forms a quaternary complex, which mediates the interaction between ILF3 and exportin-5. Later, ILF3 affinity towards VA1 is stimulated by exportin-5 that facilitates the interaction between RNA and the export receptor. These events cause co-export of both VA1 and ILF3. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| Features and Benefits: | Prestige Antibodies® are highly characterized and extensively validated antibodies with the added benefit of all available characterization data for each target being accessible via the Human Protein Atlas portal linked just below the product name at the top of this page. The uniqueness and low cross-reactivity of the Prestige Antibodies® to other proteins are due to a thorough selection of antigen regions, affinity purification, and stringent selection. Prestige antigen controls are available for every corresponding Prestige Antibody and can be found in the linkage section. Every Prestige Antibody is tested in the following ways: • IHC tissue array of 44 normal human tissues and 20 of the most common cancer type tissues. • Protein array of 364 human recombinant protein fragments. |
| General description: | ILF3 (interleukin enhancer binding factor 3) is a double-stranded nuclear RNA-binding protein. It is composed of a bipartite nuclear localization signal (NLS), an RGG (Arg-Gly-Gly) box RNA-binding motif, and two double-stranded RNA binding domains (dsRBDs) that acts as novel nuclear export sequence (NES) in intact cells. |
| Immunogen: | Interleukin enhancer-binding factor 3 recombinant protein epitope signature tag (PrEST) |
| Legal Information: | Prestige Antibodies is a registered trademark of Merck KGaA, Darmstadt, Germany |
| Other Notes: | Corresponding Antigen APREST86210 |
| Physical form: | Solution in phosphate-buffered saline, pH 7.2, containing 40% glycerol and 0.02% sodium azide |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |